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Literature summary extracted from

  • Bultsma, Y.; Keune, W.J.; Divecha, N.
    PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha (2010), Biochem. J., 430, 223-235.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.149 recombinant expression of GST-tagged isozymes and of HA-tagged isozymes in HEK-293 cells Homo sapiens
2.7.1.149 recombinant expression of Myc-tagged isozymes, co-expression of the wild-type PIP4Kalpha isozyme suppresses ubiquitination of isozyme PIP4Kbeta Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.149 additional information isozymes PIPKalpha and PIP4Kbeta inactivation by specific RNAi. Overexpression of PIP4Kalpha leads to a dramatic increase in PIP4K activity, that is not present when wild-type PIP4Kbeta kinase is expressed Homo sapiens
2.7.1.149 additional information knockdown of both isozymes, enzyme activity associated with kinase-inactive Myc-PIP4Kbeta is reduced to 24% compared with that associated with the active Myc-PIP4Kbeta Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.149 cytosol
-
 Homo sapiens 5829
-
2.7.1.149 cytosol PIPKIIbeta and PIPKIIalpha Rattus norvegicus 5829
-
2.7.1.149 nucleus isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha Homo sapiens 5634
-
2.7.1.149 nucleus isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha Rattus norvegicus 5634
-
2.7.1.149 plasma membrane
-
 Homo sapiens 5886
-
2.7.1.149 plasma membrane
-
 Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.149 Mg2+ required Homo sapiens
2.7.1.149 Mg2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.149 49000
-
 2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta Homo sapiens
2.7.1.149 97000
-
 recombinant isozyme PIP4Kbeta Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Rattus norvegicus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.149 additional information Homo sapiens isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha ?
-
 ?
2.7.1.149 additional information Rattus norvegicus isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.149 Homo sapiens
-
-
-
2.7.1.149 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.149 recombinant GST-tagged isozymes by glutathione affinity chromatography, recombinant HA-tagged isozymes by affinity chromatography from HEK-293 cells Homo sapiens
2.7.1.149 recombinant Myc-tagged isozymes by affinity chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.149 additional information isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha Homo sapiens ?
-
 ?
2.7.1.149 additional information isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.149 homodimer 2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta Homo sapiens
2.7.1.149 homodimer crystal structure of isozyme PIP4Kbeta Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.149 PIP4K
-
 Homo sapiens
2.7.1.149 PIP4K
-
 Rattus norvegicus
2.7.1.149 PIP4Kalpha
-
 Homo sapiens
2.7.1.149 PIP4Kalpha
-
 Rattus norvegicus
2.7.1.149 PIP4Kbeta
-
 Homo sapiens
2.7.1.149 PIP4Kbeta
-
 Rattus norvegicus
2.7.1.149 PtdIns5P-4-kinase
-
 Homo sapiens
2.7.1.149 PtdIns5P-4-kinase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.149 ATP
-
 Homo sapiens
2.7.1.149 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.149 physiological function isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions Homo sapiens
2.7.1.149 physiological function isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions Rattus norvegicus