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Literature summary extracted from

  • Eric Thomas, C.; Chen, Y.C.; Garcia, G.A.
    Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases (2011), Biochem. Biophys. Res. Commun., 410, 34-39.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.64 C145V/T146V mutant shows lower kcat and higher Km value compared to wild-type Escherichia coli
2.4.2.64 C145V/T146V/P147N/V217G quadruble mutant is affected least by pteridine inhibitors, mutant shows lowest kcat and highest Km value compared to wild-type and other mutants Escherichia coli
2.4.2.64 C145V/T146V/V217G triple mutant shows lowest Ki value for biopterin, mutant shows lower kcat and higher Km value compared to wild-type Escherichia coli
2.4.2.64 V217G mutant shows lower kcat and higher Km value compared to wild-type Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.64 biopterin
-
 Escherichia coli
2.4.2.64 biopterin
-
 Homo sapiens
2.4.2.64 pterin
-
 Escherichia coli
2.4.2.64 pterin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.64 0.00026
-
 queuine pH 7.3, 37°C, wild-type Homo sapiens
2.4.2.64 0.00035
-
 guanine pH 7.3, 37°C, wild-type Escherichia coli
2.4.2.64 0.00087
-
 guanine pH 7.3, 37°C, mutant C145V/T146V Escherichia coli
2.4.2.64 0.001
-
 guanine pH 7.3, 37°C, mutant V217G Escherichia coli
2.4.2.64 0.00209
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/V217G Escherichia coli
2.4.2.64 0.035
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.64 Escherichia coli
-
-
-
2.4.2.64 Homo sapiens Q9BXR0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.64 guanine34 in tRNA + queuine
-
 Escherichia coli queuine34 in tRNA + guanine
-
 r
2.4.2.64 guanine34 in tRNA + queuine
-
 Homo sapiens queuine34 in tRNA + guanine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.64 TGT
-
 Escherichia coli
2.4.2.64 TGT
-
 Homo sapiens
2.4.2.64 tRNA-guanine transglycosylase
-
 Escherichia coli
2.4.2.64 tRNA-guanine transglycosylase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.64 37
-
 assay at Escherichia coli
2.4.2.64 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.64 0.000259
-
 guanine pH 7.3, 37°C, mutant V217G Escherichia coli
2.4.2.64 0.00053
-
 guanine pH 7.3, 37°C, mutant C145V/T146V Escherichia coli
2.4.2.64 0.00062
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G Escherichia coli
2.4.2.64 0.0012
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/V217G Escherichia coli
2.4.2.64 0.00629
-
 guanine pH 7.3, 37°C, wild-type Escherichia coli
2.4.2.64 0.008
-
 queuine pH 7.3, 37°C, wild-type Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.64 7.3
-
 assay at Escherichia coli
2.4.2.64 7.3
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.64 0.00042
-
 pterin pH 7.3, 37°C, wild-type Escherichia coli
2.4.2.64 0.0009
-
 pterin pH 7.3, 37°C, mutant V217G Escherichia coli
2.4.2.64 0.0013
-
 pterin pH 7.3, 37°C, mutant C145V/T146V/V217G Escherichia coli
2.4.2.64 0.0022
-
 pterin pH 7.3, 37°C, mutant C145V/T146V Escherichia coli
2.4.2.64 0.0087
-
 biopterin pH 7.3, 37°C, wild-type Homo sapiens
2.4.2.64 0.037
-
 pterin pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G Escherichia coli
2.4.2.64 0.16
-
 biopterin pH 7.3, 37°C, mutant C145V/T146V/V217G Escherichia coli
2.4.2.64 0.55
-
 biopterin pH 7.3, 37°C, mutant C145V/T146V Escherichia coli
2.4.2.64 0.55
-
 biopterin pH 7.3, 37°C, mutant V217G Escherichia coli
2.4.2.64 1.05
-
 biopterin pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G Escherichia coli
2.4.2.64 3
-
 biopterin pH 7.3, 37°C, wild-type Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.64 0.017
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G Escherichia coli
2.4.2.64 0.26
-
 guanine pH 7.3, 37°C, mutant V217G Escherichia coli
2.4.2.64 0.58
-
 guanine pH 7.3, 37°C, mutant C145V/T146V/V217G Escherichia coli
2.4.2.64 0.61
-
 guanine pH 7.3, 37°C, mutant C145V/T146V Escherichia coli
2.4.2.64 18
-
 guanine pH 7.3, 37°C, wild-type Escherichia coli
2.4.2.64 31.6
-
 queuine pH 7.3, 37°C, wild-type Homo sapiens