EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.74 | expressed in Escherichia coli BL21(DE3) cells | Comamonas testosteroni |
1.13.11.76 | expression in Escherichia coli | Comamonas testosteroni |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.74 | Ca2+ | 79.4% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Cd2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Co2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Cu2+ | 2.9% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | EDTA | - |
Comamonas testosteroni | |
1.13.11.74 | Fe2+ | 57.4% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Fe3+ | 4.4% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | H2O2 | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
1.13.11.74 | K3Fe(CN)6 | 41.2% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Mg2+ | 91.2% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Mn2+ | 30.9% residual activity at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Ni2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
1.13.11.74 | Zn2+ | abolishes enzyme activity completely at 2 mM | Comamonas testosteroni | |
1.13.11.76 | Ca2+ | 2 mM, 79% residual activity | Comamonas testosteroni | |
1.13.11.76 | Cd2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
1.13.11.76 | Co2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
1.13.11.76 | Cu2+ | 2 mM, 2.9% residual activity | Comamonas testosteroni | |
1.13.11.76 | EDTA | 2 mM, 50% residual activity | Comamonas testosteroni | |
1.13.11.76 | Fe2+ | 2 mM, 57% residual activity. Not inhibitory in presence of ascorbate | Comamonas testosteroni | |
1.13.11.76 | H2O2 | 2 mM, complete loss of activity | Comamonas testosteroni | |
1.13.11.76 | Mg2+ | 2 mM, 91% residual activity | Comamonas testosteroni | |
1.13.11.76 | Mn2+ | 2 mM, 31% residual activity | Comamonas testosteroni | |
1.13.11.76 | Ni2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
1.13.11.76 | Zn2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.74 | 0.00077 | - |
2-amino-5-chlorophenol | in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni | |
1.13.11.74 | 0.00089 | - |
2-Aminophenol | in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni | |
1.13.11.76 | 0.00077 | - |
2-amino-5-chlorophenol | pH 8.0, 22°C | Comamonas testosteroni | |
1.13.11.76 | 0.00089 | - |
2-Aminophenol | pH 8.0, 22°C | Comamonas testosteroni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.74 | 33000 | - |
2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
1.13.11.74 | 38000 | - |
2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
1.13.11.74 | 130000 | - |
gel filtration | Comamonas testosteroni |
1.13.11.76 | 33000 | - |
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
1.13.11.76 | 38000 | - |
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
1.13.11.76 | 130000 | - |
gel filtration | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.74 | 2-amino-5-chlorophenol + O2 | Comamonas testosteroni | 68% activity compared to 2-aminophenol | ? | - |
? | |
1.13.11.74 | 2-amino-5-chlorophenol + O2 | Comamonas testosteroni CNB-1 | 68% activity compared to 2-aminophenol | ? | - |
? | |
1.13.11.74 | 2-aminophenol + O2 | Comamonas testosteroni | 100% activity | 2-aminomuconate 6-semialdehyde | - |
? | |
1.13.11.74 | 2-aminophenol + O2 | Comamonas testosteroni CNB-1 | 100% activity | 2-aminomuconate 6-semialdehyde | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.74 | Comamonas testosteroni | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
1.13.11.74 | Comamonas testosteroni CNB-1 | Q6J1Z5 and Q6J1Z6 | subunit alpha and catalytic subunit beta | - |
1.13.11.76 | Comamonas testosteroni | Q6J1Z5 | subunit alpha | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.74 | ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration | Comamonas testosteroni |
1.13.11.76 | - |
Comamonas testosteroni |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.76 | 29.7 | - |
pH 8.0, 22°C | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.74 | 2-amino-5-chlorophenol + O2 | 68% activity compared to 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
1.13.11.74 | 2-amino-5-chlorophenol + O2 | 68% activity compared to 2-aminophenol | Comamonas testosteroni CNB-1 | ? | - |
? | |
1.13.11.74 | 2-aminophenol + O2 | 100% activity | Comamonas testosteroni | 2-aminomuconate 6-semialdehyde | - |
? | |
1.13.11.74 | 2-aminophenol + O2 | 100% activity | Comamonas testosteroni CNB-1 | 2-aminomuconate 6-semialdehyde | - |
? | |
1.13.11.74 | additional information | 5% activity with catechol | Comamonas testosteroni | ? | - |
? | |
1.13.11.74 | additional information | 5% activity with catechol | Comamonas testosteroni CNB-1 | ? | - |
? | |
1.13.11.74 | protocatechuate + O2 | 33% activity compared to 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
1.13.11.74 | protocatechuate + O2 | 33% activity compared to 2-aminophenol | Comamonas testosteroni CNB-1 | ? | - |
? | |
1.13.11.76 | 2-amino-5-chlorophenol + O2 | 68% of the activity with 2-aminophenol | Comamonas testosteroni | 2-amino-5-chloromuconate 6-semialdehyde | - |
? | |
1.13.11.76 | 2-aminophenol + O2 | - |
Comamonas testosteroni | ? | - |
? | |
1.13.11.76 | catechol + O2 | 5% of the activity with 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
1.13.11.76 | additional information | no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol | Comamonas testosteroni | ? | - |
? | |
1.13.11.76 | protocatechuate + O2 | 33% of the activity with 2-aminophenol | Comamonas testosteroni | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.74 | heterotetramer | 2 * 38000 + 2 * 33000, SDS-PAGE | Comamonas testosteroni |
1.13.11.76 | tetramer | 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.76 | CnbcA | - |
Comamonas testosteroni |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.13.11.74 | 2 | - |
in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C | Comamonas testosteroni | EDTA |