EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.172 | expressed in Escherichia coli BL21(DE3) cells | Ralstonia sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.172 | 0.05641 | - |
NADPH | in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. | |
1.14.13.172 | 0.05976 | - |
NADH | in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. | |
1.14.13.172 | 0.1071 | - |
salicylate | in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. | |
1.14.13.172 | 0.3284 | - |
2-Nitrophenol | in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. | |
1.14.13.172 | 0.9398 | - |
2,6-dihydroxybenzoate | in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.172 | Iron | the enzyme contains one Rieske-type [2Fe-2S] cluster and 7.5 g iron per mol enzyme | Ralstonia sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.172 | 18800 | - |
3 * 51000 + 3 * 18800, His6-tagged enzyme, calculated from amino acid sequence | Ralstonia sp. |
1.14.13.172 | 20000 | - |
3 * 52000 + 3 * 20000, His6-tagged enzyme, SDS-PAGE | Ralstonia sp. |
1.14.13.172 | 51000 | - |
3 * 51000 + 3 * 18800, His6-tagged enzyme, calculated from amino acid sequence | Ralstonia sp. |
1.14.13.172 | 52000 | - |
3 * 52000 + 3 * 20000, His6-tagged enzyme, SDS-PAGE | Ralstonia sp. |
1.14.13.172 | 195200 | - |
gel filtration | Ralstonia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.172 | salicylate + NADH + H+ + O2 | Ralstonia sp. | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADH + H+ + O2 | Ralstonia sp. U2 | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADPH + H+ + O2 | Ralstonia sp. | - |
2,5-dihydroxybenzoate + NADP+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADPH + H+ + O2 | Ralstonia sp. U2 | - |
2,5-dihydroxybenzoate + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.172 | Ralstonia sp. | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
1.14.13.172 | Ralstonia sp. U2 | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.172 | the three-component Fe-S protein complex (NagAaGHAb) of the enzyme is purified by HisTrap column chromatography and Sephadex G-25 gel filtration | Ralstonia sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.172 | 107.1 | - |
after 124fold purification, in 50 mM MES buffer (pH 6.4), at 22°C | Ralstonia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.172 | 2,4-dihydroxybenzoate + NADH + H+ + O2 | - |
Ralstonia sp. | ? | - |
? | |
1.14.13.172 | 2,4-dihydroxybenzoate + NADH + H+ + O2 | - |
Ralstonia sp. U2 | ? | - |
? | |
1.14.13.172 | 2,6-dihydroxybenzoate + NADH + H+ + O2 | - |
Ralstonia sp. | ? | - |
? | |
1.14.13.172 | 2,6-dihydroxybenzoate + NADH + H+ + O2 | - |
Ralstonia sp. U2 | ? | - |
? | |
1.14.13.172 | 2-nitrophenol + NADH + H+ + O2 | - |
Ralstonia sp. | ? | - |
? | |
1.14.13.172 | 2-nitrophenol + NADH + H+ + O2 | - |
Ralstonia sp. U2 | ? | - |
? | |
1.14.13.172 | salicylate + NADH + H+ + O2 | - |
Ralstonia sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADH + H+ + O2 | - |
Ralstonia sp. U2 | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADPH + H+ + O2 | - |
Ralstonia sp. | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADPH + H+ + O2 | - |
Ralstonia sp. U2 | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.172 | heterohexamer | 3 * 51000 + 3 * 18800, His6-tagged enzyme, calculated from amino acid sequence | Ralstonia sp. |
1.14.13.172 | heterohexamer | 3 * 52000 + 3 * 20000, His6-tagged enzyme, SDS-PAGE | Ralstonia sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.172 | NagGH | - |
Ralstonia sp. |
1.14.13.172 | S5H | - |
Ralstonia sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.172 | NADH | - |
Ralstonia sp. | |
1.14.13.172 | NADPH | - |
Ralstonia sp. | |
1.14.13.172 | [2Fe-2S]-center | - |
Ralstonia sp. |