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Literature summary extracted from

  • Lee, W.H.; Shin, S.Y.; Kim, M.D.; Han, N.S.; Seo, J.H.
    Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli (2012), Appl. Microbiol. Biotechnol., 93, 2327-2334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.73 gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.73 additional information overexpression of Gsk in the fed-batch fermentation in recombinant Escherichia coli strain BL21star(DE3) with glucose as the sole carbon source, leads to a significant improvement of GDP-L-fucose production, profiles of cell growth and GDP-L-fucose production, overview Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.73 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.73 ATP + guanosine Escherichia coli
-
 ADP + GMP
-
 ?
2.7.1.73 ATP + inosine Escherichia coli
-
 ADP + IMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.73 Escherichia coli
-
 ATCC 10798, gene gsk
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.73 ATP + guanosine
-
 Escherichia coli ADP + GMP
-
 ?
2.7.1.73 ATP + inosine
-
 Escherichia coli ADP + IMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.73 Gsk
-
 Escherichia coli
2.7.1.73 guanosine-inosine kinase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.73 ATP
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.1.73 metabolism in the salvage pathway, guanosine and inosine are directly phosphorylated to GMP and IMP, respectively, by guanosine-inosine kinase, or guanosine is indirectly converted to GMP through serial phosphorolysis and phosphorylation catalyzed by purine nucleoside phosphorylase and guanine phosphoribosyltransferase, respectively, pathway, overview Escherichia coli