Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, Y.; Aono, T.; Poole, P.; Finan, T.M.
    NAD(P)+-malic enzyme mutants of Sinorhizobium sp. strain NGR234, but not Azorhizobium caulinodans ORS571, maintain symbiotic N2 fixation capabilities (2012), Appl. Environ. Microbiol., 78, 2803-2812.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 fumarate
-
 Azorhizobium caulinodans
1.1.1.39 fumarate
-
 Sinorhizobium sp.
1.1.1.39 succinate
-
 Azorhizobium caulinodans
1.1.1.39 succinate
-
 Sinorhizobium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.39 gene dme or azc3656, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli Sinorhizobium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.39 additional information azc3656 mutants show about 4fold reduced NAD+-malic enzyme activity Sinorhizobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 acetyl-CoA
-
 Azorhizobium caulinodans
1.1.1.39 acetyl-CoA
-
 Sinorhizobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.101
-
 NAD+ pH 7.8, 30°C, AZC3656 Sinorhizobium sp.
1.1.1.39 0.59
-
 (S)-malate pH 7.8, 30°C, AZC3656, in presence of 1 mM fumarate Sinorhizobium sp.
1.1.1.39 0.64
-
 (S)-malate pH 7.8, 30°C, AZC3656, in presence of 10 mM succinate Sinorhizobium sp.
1.1.1.39 2.1
-
 NADP+ pH 7.8, 30°C, AZC3656 Sinorhizobium sp.
1.1.1.39 2.7
-
 (S)-malate pH 7.8, 30°C, AZC3656 Sinorhizobium sp.
1.1.1.39 27.6
-
 (S)-malate pH 7.8, 30°C, AZC3656, in presence of 0.05 mM acetyl-CoA Sinorhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.39 (S)-malate + NAD(P)+ Azorhizobium caulinodans
-
 pyruvate + CO2 + NAD(P)H
-
 ?
1.1.1.39 (S)-malate + NAD+ Sinorhizobium sp.
-
 pyruvate + CO2 + NADH
-
 ?
1.1.1.39 (S)-malate + NAD+ Sinorhizobium sp. NGR234
-
 pyruvate + CO2 + NADH
-
 ?
1.1.1.39 (S)-malate + NADP+ Sinorhizobium sp.
-
 pyruvate + CO2 + NADPH
-
 ?
1.1.1.39 (S)-malate + NADP+ Sinorhizobium sp. NGR234
-
 pyruvate + CO2 + NADPH
-
 ?
1.1.1.40 (S)-malate + NADP+ Sinorhizobium meliloti
-
 pyruvate + CO2 + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Azorhizobium caulinodans
-
 gene azc3656
-
1.1.1.39 Sinorhizobium sp. B6E9W4 gene azc3656 or dme
-
1.1.1.39 Sinorhizobium sp. NGR234 B6E9W4 gene azc3656 or dme
-
1.1.1.40 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39 recombinant His-tagged enzyme AZC3656 from Escherichia coli by nickel affinity chromatography Sinorhizobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 0.201
-
 NAD+-dependent ME activity in the wild-type strain, pH 7.8, 30°C, AZC3656 Sinorhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NAD(P)+
-
 Azorhizobium caulinodans pyruvate + CO2 + NAD(P)H
-
 ?
1.1.1.39 (S)-malate + NAD+
-
 Sinorhizobium sp. pyruvate + CO2 + NADH
-
 ?
1.1.1.39 (S)-malate + NAD+
-
 Sinorhizobium sp. NGR234 pyruvate + CO2 + NADH
-
 ?
1.1.1.39 (S)-malate + NADP+
-
 Sinorhizobium sp. pyruvate + CO2 + NADPH
-
 ?
1.1.1.39 (S)-malate + NADP+
-
 Sinorhizobium sp. NGR234 pyruvate + CO2 + NADPH
-
 ?
1.1.1.40 (S)-malate + NADP+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.39 AZC3656 protein
-
 Azorhizobium caulinodans
1.1.1.39 AZC3656 protein
-
 Sinorhizobium sp.
1.1.1.39 DME
-
 Azorhizobium caulinodans
1.1.1.39 DME
-
 Sinorhizobium sp.
1.1.1.39 NAD(P)+-malic enzyme
-
 Azorhizobium caulinodans
1.1.1.39 NAD(P)+-malic enzyme
-
 Sinorhizobium sp.
1.1.1.40 NAD(P)+-malic enzyme
-
 Sinorhizobium meliloti
1.1.1.40 TME
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.39 30
-
 assay at Sinorhizobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 7.8
-
 AZC3656 shows high NAD+-ME activity at pH 7.8, with Michaelis-Menten-like kinetics, at various concentrations of malate, The enzyme exhibits only a very limited positive cooperativity with respect to malate Sinorhizobium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+
-
 Sinorhizobium sp.
1.1.1.39 NAD+ preferred cofactor Azorhizobium caulinodans
1.1.1.39 NADP+
-
 Azorhizobium caulinodans
1.1.1.39 NADP+
-
 Sinorhizobium sp.
1.1.1.40 NADP+ the enzyme is strictly NADP+-dependent Sinorhizobium meliloti

General Information

EC Number General Information Comment Organism
1.1.1.39 malfunction dme mutants of the broad-host-range Sinorhizobium sp. strain NGR234 form nodules whose level of N2 fixation vary from 27 to 83% (plant dry weight) of the wild-type level, depending on the host plant inoculated. The single dme mutant fixes N2 at reduced rate. A pckA dme double mutant has no N2-fixing activity, PCK is phosphoenolpyruvate carboxykinase. Symbiotic phenotypes of NGR234 and NGR234 dme mutants on different host plants, overview Sinorhizobium sp.
1.1.1.39 physiological function AZC3656 protein is a NAD+-malic enzyme, i.e. DME, while AZC0119 protein is not a malic enzyme. DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate. But NGR234 bacteroids appear to synthesize pyruvate from TCA cycle intermediates via DME or PCK, phosphoenolpyruvate carboxykinase, pathways, overview Sinorhizobium sp.
1.1.1.39 physiological function DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate Azorhizobium caulinodans