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Literature summary extracted from

  • Primak, Y.A.; Du, M.; Miller, M.C.; Wells, D.H.; Nielsen, A.T.; Weyler, W.; Beck, Z.Q.
    Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei (2011), Appl. Environ. Microbiol., 77, 7772-7778.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expressed in Escherichia coli BL21 Star (lambdaDE3) cells Methanosarcina mazei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 additional information the enzyme is not inhibited by dimethylallyl diphosphate, geranyl diphosphate, farnesyl diphosphate, isopentenyl monophosphate, and diphosphomevalonate Methanosarcina mazei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.068
-
 (R)-mevalonate apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei
2.7.1.36 0.464
-
 ATP apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 35500
-
 2 * 35500, calculated from amino acid sequence Methanosarcina mazei
2.7.1.36 78000
-
 gel filtration Methanosarcina mazei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanosarcina mazei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 HiTrap IMAC column chromatography and HiTrap Q column chromatography Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Methanosarcina mazei ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 homodimer 2 * 35500, calculated from amino acid sequence Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 MVK
-
 Methanosarcina mazei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.36 4.3
-
 (R)-mevalonate in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C Methanosarcina mazei