BRENDA - Enzyme Database

Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus

Van der Borght, J.; Chen, C.; Hoflack, L.; Van Renterghem, L.; Desmet, T.; Soetaert, W.; Appl. Environ. Microbiol. 77, 6939-6944 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.64
expressed in Escherichia coli XL10 Gold cells
Caldanaerobacter subterraneus subsp. pacificus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.64
D-glucose
strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM
Caldanaerobacter subterraneus subsp. pacificus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.64
3.5
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
20.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
64.8
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
171.5
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
183.4
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.64
90487
-
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.64
Caldanaerobacter subterraneus subsp. pacificus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.64
Ni-NTA agarose resin column chromatography
Caldanaerobacter subterraneus subsp. pacificus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.64
alpha,alpha-trehalose + phosphate
specific substrate
721346
Caldanaerobacter subterraneus subsp. pacificus
beta-D-glucose 1-phosphate + D-glucose
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-allose
4.5% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-arabinose
1.8% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-fucose
22% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-galactose
83.7% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-lyxose
1.3% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-mannose
14.1% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-xylose
135.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-L-fucose
109.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + beta-L-arabinose
79.9% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + D-glucose
100% activity with D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
alpha,alpha-trehalose + phosphate
-
-
-
r
2.4.1.64
additional information
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.64
?
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.64
80
-
-
Caldanaerobacter subterraneus subsp. pacificus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.1.64
65
-
no loss of activity after 1 h of incubation at 65°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
72
-
50% residual activity remains after 1 h of incubation at 72°C
Caldanaerobacter subterraneus subsp. pacificus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.64
51.4
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
54
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
59
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
64.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
68.1
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.64
5.5
-
the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
7
-
the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis
Caldanaerobacter subterraneus subsp. pacificus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.64
4.5
7
activity is over 90% in the range of pH 4.5 to 7.0
Caldanaerobacter subterraneus subsp. pacificus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.4.1.64
Caldanaerobacter subterraneus subsp. pacificus
calculated from amino acid sequence
-
5.4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.64
expressed in Escherichia coli XL10 Gold cells
Caldanaerobacter subterraneus subsp. pacificus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.64
D-glucose
strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM
Caldanaerobacter subterraneus subsp. pacificus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.64
3.5
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
20.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
64.8
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
171.5
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
183.4
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.64
90487
-
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.64
Ni-NTA agarose resin column chromatography
Caldanaerobacter subterraneus subsp. pacificus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.64
alpha,alpha-trehalose + phosphate
specific substrate
721346
Caldanaerobacter subterraneus subsp. pacificus
beta-D-glucose 1-phosphate + D-glucose
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-allose
4.5% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-arabinose
1.8% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-fucose
22% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-galactose
83.7% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-lyxose
1.3% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-mannose
14.1% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-D-xylose
135.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + alpha-L-fucose
109.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + beta-L-arabinose
79.9% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
2.4.1.64
beta-D-glucose 1-phosphate + D-glucose
100% activity with D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
alpha,alpha-trehalose + phosphate
-
-
-
r
2.4.1.64
additional information
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.64
?
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.64
80
-
-
Caldanaerobacter subterraneus subsp. pacificus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.1.64
65
-
no loss of activity after 1 h of incubation at 65°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
72
-
50% residual activity remains after 1 h of incubation at 72°C
Caldanaerobacter subterraneus subsp. pacificus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.64
51.4
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
54
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
59
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
64.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
68.1
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.64
5.5
-
the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
7
-
the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis
Caldanaerobacter subterraneus subsp. pacificus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.64
4.5
7
activity is over 90% in the range of pH 4.5 to 7.0
Caldanaerobacter subterraneus subsp. pacificus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.4.1.64
Caldanaerobacter subterraneus subsp. pacificus
calculated from amino acid sequence
-
5.4
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.1.64
0.315
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
0.371
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
0.793
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
3.17
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
17
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.1.64
0.315
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
0.371
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
0.793
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
3.17
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
2.4.1.64
17
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus