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Literature summary extracted from

  • Comfort, D.A.; Chou, C.J.; Conners, S.B.; VanFossen, A.L.; Kelly, R.M.
    Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus (2008), Appl. Environ. Microbiol., 74, 1281-1283.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 0.0827
-
 4-nitrophenyl alpha-maltopyranoside pH 7.0, 90°C Pyrococcus furiosus
3.2.1.48 0.051
-
 4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 3.2
-
 panose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 5
-
 isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 7.1
-
 maltose 90°C, pH not specified in the publication Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.2 additional information the presence or absence of various divalent cations or EDTA had a minimal effect on enzyme activity Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 additional information Pyrococcus furiosus pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Pyrococcus furiosus Q8U2G5
-
-
3.2.1.48 Pyrococcus furiosus Q8U4F6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.48 native enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 4-nitrophenyl alpha-D-galactoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
3.2.1.2 4-nitrophenyl alpha-maltopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-maltopyranose
-
 ?
3.2.1.2 glycogen + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?
3.2.1.2 maltose + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?
3.2.1.2 additional information the enzyme does not hydrolyze starch, glycogen, pullulan or large maltooligosaccharides Pyrococcus furiosus ?
-
 ?
3.2.1.2 starch + H2O the enzyme is specific for short alpha-glucans. Similar responses to maltose, glycogen, and starch but not to pullulan Pyrococcus furiosus ?
-
 ?
3.2.1.48 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.48 isomaltose + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.48 maltose + H2O comparable catalytic efficiencies for panose and maltose Pyrococcus furiosus ?
-
 ?
3.2.1.48 additional information pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport Pyrococcus furiosus ?
-
 ?
3.2.1.48 panose + H2O comparable catalytic efficiencies for panose and maltose Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.2 beta-amylase
-
 Pyrococcus furiosus
3.2.1.2 PF0870
-
 Pyrococcus furiosus
3.2.1.48 isomaltase
-
 Pyrococcus furiosus
3.2.1.48 PF0132
-
 Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.2 962
-
 4-nitrophenyl alpha-maltopyranoside pH 7.0, 90°C Pyrococcus furiosus
3.2.1.48 21.9
-
 4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 58.8
-
 isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 278
-
 panose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 1600
-
 maltose 90°C, pH not specified in the publication Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
3.2.1.48 physiological function involved in processing alpha-1,6-glucans. Pullulan is likely degraded extracellularly by an amylopullulanase and further hydrolyzed by the PF0132 protein after intracellular transport Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.48 11.8
-
 isomaltose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 85.6
-
 panose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 225
-
 maltose 90°C, pH not specified in the publication Pyrococcus furiosus
3.2.1.48 429
-
 4-nitrophenyl alpha-D-glucopyranoside 90°C, pH not specified in the publication Pyrococcus furiosus