Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gao, J.; Bauer, M.W.; Shockley, K.R.; Pysz, M.A.; Kelly, R.M.
    Growth of hyperthermophilic archaeon Pyrococcus furiosus on chitin involves two family 18 chitinases (2003), Appl. Environ. Microbiol., 69, 3119-3128.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli Pyrococcus furiosus
3.2.1.200 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.200 0.13
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 0.24
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 0.3
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 39700
-
 x * 39700, calculated from sequence Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pyrococcus furiosus Q8U1H4
-
-
3.2.1.200 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O the enzyme is twice as active on colloidal chitin than on untreted chitin. It cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly Pyrococcus furiosus ?
-
 ?
3.2.1.14 crab shell chitin + H2O the enzyme cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly Pyrococcus furiosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.14 additional information no activity toward 4-nitrophenyl-N-acetylglucosamine and chitooligomers smaller than chitotetraose Pyrococcus furiosus ?
-
 ?
3.2.1.200 colloidal chitin + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 crab shell chitin + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O ChiBcat hydrolyzes chitooligosaccharides (degree of polymerization = 3 to 6), yielding predominantly chitobiose. (GlcNAc)6 is processively degraded to mostly (GlcNAc)4 and (GlcNAc)2, with limited formation of (GlcNAc)3 Pyrococcus furiosus N,N'-diacetylchitobiose + GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta
-
 ?
3.2.1.200 additional information no activity with chitobiose and 4-nitrophenyl-N-acetylglucosamine Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 39700, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA
-
 Pyrococcus furiosus
3.2.1.200 ChiB
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.200 95
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 101
-
 melting temperature Pyrococcus furiosus
3.2.1.200 114
-
 melting temperature Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Pyrococcus furiosus
3.2.1.200 6
-
-
 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.200 2.15
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 2.56
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 5.89
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.200 8580
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 13380
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
3.2.1.200 13490
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus