BRENDA - Enzyme Database

Cloning, expression and characterization of NADP-dependent isocitrate dehydrogenase from Staphylococcus aureus

Prasad, U.V.; Vasu, D.; Kumar, Y.N.; Kumar, P.S.; Yeswanth, S.; Swarupa, V.; Phaneendra, B.V.; Chaudhary, A.; Sarma, P.V.; Appl. Biochem. Biotechnol. 169, 862-869 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
Staphylococcus aureus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0082
-
isocitrate
recombinant enzyme, pH 7.5, 30C
Staphylococcus aureus
1.1.1.42
0.0675
-
NADP+
recombinant enzyme, pH 7.5, 30C
Staphylococcus aureus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Staphylococcus aureus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
40000
-
x * 40000, SDS-PAGE
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Staphylococcus aureus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Staphylococcus aureus ATCC 12600
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Staphylococcus aureus
D7RIE8
-
-
1.1.1.42
Staphylococcus aureus ATCC 12600
D7RIE8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
721312
Staphylococcus aureus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
721312
Staphylococcus aureus ATCC 12600
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 40000, SDS-PAGE
Staphylococcus aureus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
30
-
assay at
Staphylococcus aureus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
assay at
Staphylococcus aureus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
dependent on
Staphylococcus aureus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
Staphylococcus aureus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
dependent on
Staphylococcus aureus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0082
-
isocitrate
recombinant enzyme, pH 7.5, 30C
Staphylococcus aureus
1.1.1.42
0.0675
-
NADP+
recombinant enzyme, pH 7.5, 30C
Staphylococcus aureus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Staphylococcus aureus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
40000
-
x * 40000, SDS-PAGE
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Staphylococcus aureus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Staphylococcus aureus ATCC 12600
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
721312
Staphylococcus aureus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
721312
Staphylococcus aureus ATCC 12600
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 40000, SDS-PAGE
Staphylococcus aureus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
30
-
assay at
Staphylococcus aureus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
assay at
Staphylococcus aureus
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
isocitrate dehydrogenase plays pivotal role in the growth and pathogenesis of the bacteria
Staphylococcus aureus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
isocitrate dehydrogenase plays pivotal role in the growth and pathogenesis of the bacteria
Staphylococcus aureus