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Literature summary extracted from
- Cloning, expression and characterization of NADP-dependent isocitrate dehydrogenase from Staphylococcus aureus (2013), Appl. Biochem. Biotechnol., 169, 862-869.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.42 | gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha | Staphylococcus aureus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.42 | 0.0082 | - |
isocitrate | recombinant enzyme, pH 7.5, 30°C | Staphylococcus aureus |  |
| 1.1.1.42 | 0.0675 | - |
NADP+ | recombinant enzyme, pH 7.5, 30°C | Staphylococcus aureus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.42 | cytosol | - |
Staphylococcus aureus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 40000 | - |
x * 40000, SDS-PAGE | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NADP+ | Staphylococcus aureus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| 1.1.1.42 | isocitrate + NADP+ | Staphylococcus aureus ATCC 12600 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.42 | Staphylococcus aureus | D7RIE8 | - |
- |
| 1.1.1.42 | Staphylococcus aureus ATCC 12600 | D7RIE8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.42 | recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NADP+ | - |
Staphylococcus aureus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| 1.1.1.42 | isocitrate + NADP+ | - |
Staphylococcus aureus ATCC 12600 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | ? | x * 40000, SDS-PAGE | Staphylococcus aureus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | IDH | - |
Staphylococcus aureus |
| 1.1.1.42 | NADP-dependent isocitrate dehydrogenase | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 30 | - |
assay at | Staphylococcus aureus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 7.5 | - |
assay at | Staphylococcus aureus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.42 | NADP+ | dependent on | Staphylococcus aureus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | physiological function | isocitrate dehydrogenase plays pivotal role in the growth and pathogenesis of the bacteria | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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