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Literature summary extracted from

  • Fan, Y.Q.; Liu, H.J.; Li, C.; Luan, Y.S.; Yang, J.M.; Wang, Y.L.
    Inactivation of recombinant human brain-type creatine kinase during denaturation by guanidine hydrochloride in a macromolecular crowding system (2013), Appl. Biochem. Biotechnol., 169, 268-280.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli Homo sapiens

General Stability

EC Number General Stability Organism
2.7.3.2 polyethylene glycol 2000 (PEG 2000) and dextran 70 are used as model crowding agents to examine the effects of macromolecular crowding on the inactivation of recombinant HBCK (rHBCK) during denaturation by GdnHCl. Both PEG 2000 and dextran 70 have a protective effect on the inactivation of rHBCK induced by GdnHCl at 25°C. The presence of PEG 2000 results in the retention of 35.33% of rHBCK activity after 4 h of inactivation, while no rHBCK activity is observed after denaturation in the absence of macromolecular crowding agents. The presence of PEG 2000 and dextran 70 at a concentration of 100 g/l can decelerate the k2 value of the slow track to 21 and 33%, respectively, in comparison to values obtained in the absence of crowding agents Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine
-
 Homo sapiens ATP + creatine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 recombinant human brain-type creatine kinase
-
 Homo sapiens
2.7.3.2 rHBCK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.2 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 8
-
 assay at Homo sapiens