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Literature summary extracted from

  • Shakya, T.; Wright, G.D.
    Nucleotide selectivity of antibiotic kinases (2010), Antimicrob. Agents Chemother., 54, 1909-1913.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.95 analysis competitive assay that mimics in vivo nucleotide triphosphate concentrations and usage by the enzyme. Downstream analysis of reaction products by high-performance liquid chromatography enables the determination of partitioning of phosphate flux from nucleotide triphosphate donors to antibiotics Enterococcus casseliflavus
2.7.1.136 analysis competitive assay that mimics in vivo nucleotide triphosphate concentrations and usage by the enzyme. Downstream analysis of reaction products by high-performance liquid chromatography enables the determination of partitioning of phosphate flux from nucleotide triphosphate donors to antibiotics Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.95 Adenylyl imidodiphosphate
-
 Enterococcus casseliflavus
2.7.1.95 additional information not inhibitory: guanylyl imidodiphosphate Enterococcus casseliflavus
2.7.1.136 Adenylyl imidodiphosphate
-
 Escherichia coli
2.7.1.136 additional information not inhibitory: guanylyl imidodiphosphate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.95 0.0106
-
 ATP pH 7.5, 37°C Enterococcus casseliflavus
2.7.1.136 0.0106
-
 ATP pH 7.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.136 additional information Escherichia coli under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.95 Enterococcus casseliflavus
-
-
-
2.7.1.136 Escherichia coli Q47396
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.95 ATP + neomycin
-
 Enterococcus casseliflavus ADP + neomycin 3'-phosphate
-
 ?
2.7.1.95 additional information under physiologic concentrationsof NTPs, enzyme exclusively uses ATP Enterococcus casseliflavus ?
-
 ?
2.7.1.136 ATP + azithromycin
-
 Escherichia coli ADP + azithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + clarithromycin
-
 Escherichia coli ADP + clarithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + erythromycin
-
 Escherichia coli ADP + erythromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + neomycin
-
 Escherichia coli ADP + ?
-
 ?
2.7.1.136 ATP + spiramycin
-
 Escherichia coli ADP + spiramycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + telithromycin
-
 Escherichia coli ADP + telithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + tylosin
-
 Escherichia coli ADP + tylosin 2'-phosphate
-
 ?
2.7.1.136 additional information under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.95 aph(3'')-IIIa
-
 Enterococcus casseliflavus
2.7.1.136 macrolide 2'-phosphotransferase
-
 Escherichia coli
2.7.1.136 mphA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.95 1.59
-
 ATP pH 7.5, 37°C Enterococcus casseliflavus
2.7.1.136 1.59
-
 ATP pH 7.5, 37°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.95 0.0142
-
 Adenylyl imidodiphosphate pH 7.5, 37°C Enterococcus casseliflavus
2.7.1.136 0.0142
-
 Adenylyl imidodiphosphate pH 7.5, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.95 150
-
 ATP pH 7.5, 37°C Enterococcus casseliflavus
2.7.1.136 150
-
 ATP pH 7.5, 37°C Escherichia coli