BRENDA - Enzyme Database

Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae

Kumagai, T.; Koyama, Y.; Oda, K.; Noda, M.; Matoba, Y.; Sugiyama, M.; Antimicrob. Agents Chemother. 54, 1132-1139 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.25
expression in Escherichia coli
Streptomyces lavendulae
5.1.1.19
-
Streptomyces lavendulae subsp. lavendulae
6.3.3.5
-
Streptomyces lavendulae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.99.3
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
L-ornithine + hydroxyurea
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
L-ornithine + hydroxyurea
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.99.3
Streptomyces lavendulae
D2Z027
-
-
2.6.99.3
Streptomyces lavendulae ATCC 11924
D2Z027
-
-
3.5.3.25
Streptomyces lavendulae
D2Z025
-
-
3.5.3.25
Streptomyces lavendulae ATCC 11924
D2Z025
-
-
5.1.1.19
Streptomyces lavendulae subsp. lavendulae
D2Z026
-
-
6.3.3.5
Streptomyces lavendulae
D2Z030
-
-
6.3.3.5
Streptomyces lavendulae ATCC 11924
D2Z030
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.25
-
Streptomyces lavendulae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.99.3
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
L-arginine is not a substrate
721300
Streptomyces lavendulae
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
L-arginine is not a substrate
721300
Streptomyces lavendulae ATCC 11924
L-ornithine + hydroxyurea
-
-
-
?
5.1.1.19
O-ureido-L-serine
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
-
-
?
6.3.3.5
O-ureido-D-serine + ATP + H2O
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
D-cycloserine + CO2 + ammonia + ADP + phosphate
-
-
-
?
6.3.3.5
O-ureido-D-serine + ATP + H2O
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
D-cycloserine + CO2 + ammonia + ADP + phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.25
37
-
assay at
Streptomyces lavendulae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.25
8
-
assay at
Streptomyces lavendulae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.25
expression in Escherichia coli
Streptomyces lavendulae
5.1.1.19
-
Streptomyces lavendulae subsp. lavendulae
6.3.3.5
-
Streptomyces lavendulae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.99.3
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
O-ureido-L-serine + acetate
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
Streptomyces lavendulae
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
L-ornithine + hydroxyurea
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
Streptomyces lavendulae ATCC 11924
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
L-ornithine + hydroxyurea
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.25
-
Streptomyces lavendulae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.99.3
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
L-arginine is not a substrate
721300
Streptomyces lavendulae
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
721300
Streptomyces lavendulae ATCC 11924
L-ornithine + hydroxyurea
-
-
-
?
3.5.3.25
Nomega-hydroxy-L-arginine + H2O
L-arginine is not a substrate
721300
Streptomyces lavendulae ATCC 11924
L-ornithine + hydroxyurea
-
-
-
?
5.1.1.19
O-ureido-L-serine
O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
-
-
?
6.3.3.5
O-ureido-D-serine + ATP + H2O
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae
D-cycloserine + CO2 + ammonia + ADP + phosphate
-
-
-
?
6.3.3.5
O-ureido-D-serine + ATP + H2O
O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate
721300
Streptomyces lavendulae ATCC 11924
D-cycloserine + CO2 + ammonia + ADP + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.25
37
-
assay at
Streptomyces lavendulae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.25
8
-
assay at
Streptomyces lavendulae
General Information
EC Number
General Information
Commentary
Organism
3.5.3.25
physiological function
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
Streptomyces lavendulae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.3.25
physiological function
the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species
Streptomyces lavendulae