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Literature summary extracted from

  • Chen, Y.; Yurek, D.A.; Yu, L.; Wang, H.; Ehsani, M.E.; Qian, Y.W.; Konrad, R.J.; Jiang, X.C.; Kuo, M.S.; Cao, G.; Wang, J.
    Development of a quantitative biochemical and cellular sphingomyelin synthase assay using mass spectrometry (2013), Anal. Biochem., 438, 61-66.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.27 expressed in in Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.27 D609
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.27 0.00538
-
 C8-ceramide isoform SMS1, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens
2.7.8.27 0.005549
-
 C8-ceramide isoform SMS1, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens
2.7.8.27 0.006277
-
 C6-ceramide isoform SMS2, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens
2.7.8.27 0.007235
-
 C6-ceramide isoform SMS2, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.27 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.27 54000
-
 x * 54000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.27 phosphatidylcholine + ceramide Homo sapiens
-
 sphingomyelin + 1,2-diacyl-sn-glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.27 Homo sapiens Q86VZ5 isoform SMS1
-
2.7.8.27 Homo sapiens Q8NHU3 isoform SMS2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.27 phosphatidylcholine + C6-ceramide
-
 Homo sapiens C6-sphingomyelin + 1,2-diacyl-sn-glycerol
-
 ?
2.7.8.27 phosphatidylcholine + C8-ceramide
-
 Homo sapiens C7-sphingomyelin + 1,2-diacyl-sn-glycerol
-
 ?
2.7.8.27 phosphatidylcholine + ceramide
-
 Homo sapiens sphingomyelin + 1,2-diacyl-sn-glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.27 ? x * 54000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.8.27 SMS1 isoform Homo sapiens
2.7.8.27 SMS2 isoform Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.8.27 0.622
-
 isoform SMS1, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens D609
2.7.8.27 1.065
-
 isoform SMS2, in 50 mM Tris–HCl (pH 7.4), at 22°C Homo sapiens D609