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Literature summary extracted from

  • Morita, H.; Tanio, M.; Kondo, S.; Kato, R.; Wanibuchi, K.; Noguchi, H.; Sugio, S.; Abe, I.; Kohno, T.
    Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone (2008), Acta Crystallogr. Sect. F, 64, 304-306.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.212 purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution Rheum palmatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.212 43000
-
 2 * 43000, SDS-PAGE Rheum palmatum
2.3.1.212 83000
-
 gel filtration Rheum palmatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O Rheum palmatum
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.212 Rheum palmatum Q94FV7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rheum palmatum 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one Rheum palmatum 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.212 homodimer 2 * 43000, SDS-PAGE Rheum palmatum
2.3.1.212 More structure-function relationship, overview Rheum palmatum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.212 bAS
-
 Rheum palmatum

General Information

EC Number General Information Comment Organism
2.3.1.212 evolution the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily Rheum palmatum
2.3.1.212 additional information structure-function relationship, overview Rheum palmatum