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Literature summary extracted from

  • Fukunaga, R.; Yokoyama, S.
    Crystallization and preliminary X-ray crystallographic study of leucyl-tRNA synthetase from the archaeon Pyrococcus horikoshii (2004), Acta Crystallogr. Sect. D, 60, 1916-1918.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 overexpressed in a C-terminally truncated form in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.4 hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = b = 186.20, c = 91.43 A, alpha = beta = 90, gamma = 120°. The asymmetric unit contains one molecule of LeuRS, with a corresponding crystal volume per protein weight of 3.2 A3 Da(-1) and a solvent content of 60.7%. A data set diffracting to 2.2 A resolution is collected from a single crystal at -173°C. Selenomethionine-substituted protein crystals are prepared in order to solve the structure by the SAD phasing method Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 113000
-
 x * 113000, calculated from sequence Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Pyrococcus horikoshii

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 113000, calculated from sequence Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
 Pyrococcus horikoshii
6.1.1.4 LeuRS
-
 Pyrococcus horikoshii