EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.102 | gene cloned into pET21b (Novagen), without a stop codon, to incorporate a C-terminal His6-tag and transformed Escherichia coli strain BL21(DE3)pLysS (Stratagene) | Nicotiana tabacum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.1.102 | determination of the X-ray crystal structures of holo-, binary (NADP(H)-bound), and ternary (NADP+ and 4-hydroxy-3-methoxycinnamaldehyde-bound) NtDBR complexes | Nicotiana tabacum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.102 | (2E)-3-methylpent-2-enal | substrate inhibition | Nicotiana tabacum | |
1.3.1.102 | 1-octen-3-one | substrate inhibition | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | 0.0058 | - |
NADPH | app. Km value, pH 5.4 and 25°C, with 1-nitrocyclohexene | Nicotiana tabacum | |
1.3.1.102 | 0.057 | - |
1-nitrocyclohexene | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 0.4 | - |
1-nitrocyclohexene | above value, app. Km value, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 0.516 | - |
trans-cinnamaldehyde | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 0.837 | - |
(2E)-3-methylpent-2-enal | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 1 | - |
trans-cinnamaldehyde | above value, app. Km value, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 1.032 | - |
(2E)-3-methylpent-2-enal | app. Km value, pH 5.4 and 25°C | Nicotiana tabacum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.102 | Nicotiana tabacum | Q9SLN8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.102 | (2E)-3-methylpent-2-enal + NADPH + H+ | - |
Nicotiana tabacum | 2-methylpentanal + NADP+ | - |
? | |
1.3.1.102 | 1-cyclohexene-1-carboxaldehyde + NADPH + H+ | low yields | Nicotiana tabacum | cyclohexanecarbaldehyde + NADP+ | - |
? | |
1.3.1.102 | 1-nitrocyclohexene + NADPH + H+ | - |
Nicotiana tabacum | nitrocyclohexane + NADP+ | - |
? | |
1.3.1.102 | 1-octen-3-one + NADPH + H+ | - |
Nicotiana tabacum | octan-3-one + NADP+ | - |
? | |
1.3.1.102 | citral + NADPH + H+ | low yields | Nicotiana tabacum | ? | - |
? | |
1.3.1.102 | trans-2-nonenal + NADPH + H+ | - |
Nicotiana tabacum | nonanal + NADP+ | - |
? | |
1.3.1.102 | trans-cinnamaldehyde + NADPH + H+ | - |
Nicotiana tabacum | 3-phenylpropanal + NADP+ | - |
? | |
1.3.1.102 | [(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH + H+ | optically pure form of compound | Nicotiana tabacum | [(2R)-1-nitropropan-2-yl]benzene + NADP+ | - |
? | |
1.3.1.102 | [(1Z)-1-nitroprop-1-en-2-yl]benzene + NADPH + H+ | poor enantioselectivity | Nicotiana tabacum | [(2S)-1-nitropropan-2-yl]benzene + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.102 | homodimer | each monomer is composed of two domains, a substrate binding or catalytic domain (residues 1-135 and 304-343) and a nucleotide-binding domain (residues 136-303) | Nicotiana tabacum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.102 | NtDBR | - |
Nicotiana tabacum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | 0.52 | - |
1-octen-3-one | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 0.66 | - |
(2E)-3-methylpent-2-enal | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 0.87 | - |
trans-2-nonenal | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 1.1 | - |
trans-cinnamaldehyde | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 1.17 | - |
1-octen-3-one | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 1.3 | - |
NADPH | app. Kcat, pH 7.3 and 25°C, with 1-nitrocyclohexene | Nicotiana tabacum | |
1.3.1.102 | 1.46 | - |
1-nitrocyclohexene | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 2.92 | - |
trans-2-nonenal | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 3.5 | - |
trans-cinnamaldehyde | value above, app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 9.2 | - |
(2E)-3-methylpent-2-enal | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 11 | - |
1-nitrocyclohexene | value above, app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.102 | 5.4 | - |
with 1-nitrocyclohexene | Nicotiana tabacum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.102 | NADPH | more than 1600fold higher preference for NADPH compared with NADH | Nicotiana tabacum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | 2.6 | - |
(2E)-3-methylpent-2-enal | app. Ki value, pH 5.4 and 25°C | Nicotiana tabacum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | 2.1 | - |
trans-cinnamaldehyde | pH 7.3 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 2.8 | - |
trans-cinnamaldehyde | pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 16 | - |
1-nitrocyclohexene | pH 5.4 and 25°C | Nicotiana tabacum | |
1.3.1.102 | 25.6 | - |
1-nitrocyclohexene | pH 7.3 and 25°C | Nicotiana tabacum |