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Literature summary extracted from

  • Kato, M.; Kitao, N.; Ishida, M.; Morimoto, H.; Irino, F.; Mizuno, K.
    Expression for caffeine biosynthesis and related enzymes in Camellia sinensis (2010), Z. Naturforsch. C, 65c, 245-256.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.160 Camellia sinensis
-
 cv. Yabukita, TCS gene
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.160 flower TCS transcripts content remains constant throughout fl ower development Camellia sinensis
-
2.1.1.160 leaf TCS transcripts content is highest in young leaves and declines markedly during leaf development Camellia sinensis
-
2.1.1.160 additional information expression profi le of the tea caffeine synthasegene in developing leaves and fl owers by northern blot RNA analysis Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 7-methylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.160 TCS
-
 Camellia sinensis
2.1.1.160 tea caffeine synthase
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.160 S-adenosyl-L-methionine
-
 Camellia sinensis

Expression

EC Number Organism Comment Expression
2.1.1.160 Camellia sinensis drought stress suppresses TCS gene expression in leaves down
2.1.1.160 Camellia sinensis environmental stresses other than heavy metal stress and plant hormone treatments have no effect on the expression of TCS gene additional information
2.1.1.160 Camellia sinensis TCS transcript increases slightly on supply of a nitrogen source up

General Information

EC Number General Information Comment Organism
2.1.1.160 metabolism caffeine synthase catalyzes the S-adenosyl-L-methionine-dependent N-3- and N-1-methylation of the purine base to form caffeine, the last step in the purine alkaloid biosynthetic pathway. Pathways for the biosynthesis of caffeine and phenylpropanoids, overview Camellia sinensis
2.1.1.160 additional information regulation of TCS gene expression Camellia sinensis