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Literature summary extracted from

  • Benitez-Paez, A.; Villarroya, M.; Armengod, M.E.
    Regulation of expression and catalytic activity of Escherichia coli RsmG methyltransferase (2012), RNA, 18, 795-806.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.170 additional information activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.170 gene rsmG is the second member in a bicistronic operon, rsmG also has its own promoter, RsmG expression might depend on the activity of an inverted repeated region, located between the rsmG promoter and ribosome binding site, which works as a weak transcriptional terminator. Expression of C-terminally His6-tagged and FLAG-tagged RsmG in Escherichia coli TOP10 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.170 D56A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 D71A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
2.1.1.170 D96A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
2.1.1.170 G73A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-sensitive and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 G75A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 G77A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
2.1.1.170 H53A site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 K100A/R101A site-directed mutagenesis of the RNA-binding residues, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
2.1.1.170 K165A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 additional information a rsmG null mutant is streptomycin-resistant Escherichia coli
2.1.1.170 P79A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 R123A site-directed mutagenesis of the RNA-binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 R139A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
2.1.1.170 R139K site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
2.1.1.170 R197A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.170 Escherichia coli P0A6U5 gene rsmG encoded in the mnmG-rsmG operon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.170 recombinant FLAG-tagged RsmG from Escherichia coli TOP10 cells by affinity chromatography Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.170 cell culture activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose Escherichia coli
-

Synonyms

EC Number Synonyms Comment Organism
2.1.1.170 rsmG
-
 Escherichia coli
2.1.1.170 RsmG methyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.170 37
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.170 S-adenosyl-L-methionine
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
2.1.1.170 Escherichia coli RsmG levels decrease under conditions that down-regulate rRNA synthesis, but coordination between rRNA and RsmG expression does not seem to occur at the level of transcription initiation down
2.1.1.170 Escherichia coli slight induction of rsmGp during stationary phase is independent of the stress-inducible sigma factor RpoS up

General Information

EC Number General Information Comment Organism
2.1.1.170 malfunction loss of the m7G527 modification confers low-level streptomycin resistance and may affect ribosomal functioning Escherichia coli
2.1.1.170 additional information positively charged residues on the protein surface around the active site, K100/R101, R123, K165, and R197, might play a role in the binding of the incoming 530 loop since their change to alanine impairs the modification function of RsmG Escherichia coli
2.1.1.170 physiological function RsmG is an S-adenosyl-L-methionine-dependent methyltransferase responsible for the synthesis of m7G527 in the 530 loop of bacterial 16S rRNA. This loop is universally conserved, plays a key role in ribosomal accuracy, and is a target for streptomycin binding, mechanisms controlling RsmG expression and activity, overview. Gene rsmG as part of a bicistronic operon also has its own promoter, which appears, in actively growing cells, as a control device to offset both the relatively low stability of RsmG and inhibition of the operon promoter. Critical importance of some residues located in the active site of Escherichia coli RsmG for the m7G modification process, the residues play a role in rRNA binding and catalysis Escherichia coli