EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.1.4 | motor domain (Leu363-Arg709) expressed in Escherichia coli BL21(DE3)-RIL CodonPlus | Eremothecium gossypii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.6.1.4 | motor domain MgADP complex, hanging drop vapor diffusion method, room temperature, 14% PEG 3350, pH 8.5 | Eremothecium gossypii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.1.4 | 0.0016 | - |
ATP | motor domain, 0 mM potassium acetate, pH 6.9, 22°C | Eremothecium gossypii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.4 | Eremothecium gossypii | - |
ATCC 10895 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.1.4 | - |
Eremothecium gossypii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Eremothecium gossypii | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.1.4 | AgKar3 | - |
Eremothecium gossypii |
5.6.1.4 | Kar3-like kinesin | - |
Eremothecium gossypii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.1.4 | additional information | - |
ATP | addition of the ATP analog adenylyl-imidodiphosphate (AMPPNP, 1 mM) dramatically increases the microtuble affinity | Eremothecium gossypii | |
5.6.1.4 | 0.16 | - |
ATP | motor domain, 25 mM potassium acetate, Vmax/2 at 0.0007 mM microtubles, pH 6.9, 22°C | Eremothecium gossypii | |
5.6.1.4 | 0.17 | - |
ATP | motor domain, 0 mM potassium acetate, Vmax/2 at 0.00008 mM microtubles, pH 6.9, 22°C | Eremothecium gossypii |