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Literature summary extracted from

  • Ho, Y.Y.; Hsieh, H.C.; Huang, C.Y.
    Biochemical characterization of allantoinase from Escherichia coli BL21 (2011), Protein J., 30, 384-394.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.5 D315A complete loss of activity Escherichia coli
3.5.2.5 H186A complete loss of activity Escherichia coli
3.5.2.5 H242A complete loss of activity Escherichia coli
3.5.2.5 H59A complete loss of activity Escherichia coli
3.5.2.5 H61A complete loss of activity Escherichia coli
3.5.2.5 K146A complete loss of activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.5 5.5
-
 (S)-allantoin Co2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 6.2
-
 (S)-allantoin Mn2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 7.3
-
 (S)-allantoin Ni2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 9.6
-
 (S)-allantoin Zn2+-activated enzyme, pH 8.0, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.5 57150
-
 gel filtration Escherichia coli
3.5.2.5 93600
-
 gel filtration Escherichia coli
3.5.2.5 189500
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.5 Escherichia coli A0A140NCJ5
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.2.5 purified recombinant ALLase exhibits no activity but can be activated when preincubating with some metal ions before analyzing its activity. Renaturation follows in decreasing order Mn2+, Co2+, Zn2+, Ni2+, Cd2+, Mg2+ Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.5 1.3
-
 Ni2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 2.6
-
 Zn2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 8
-
 Co2+-activated enzyme, pH 8.0, 25°C Escherichia coli
3.5.2.5 88.2
-
 Mn2+-activated enzyme, pH 8.0, 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.5 (S)-allantoin + H2O
-
 Escherichia coli allantoate
-
 ?
3.5.2.5 additional information no substrate: hydantoin, dihydrouracil, phthalimide, dihydroorotate, and 3-imonoisoindolinone Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.5 More gel filtration chromatography reveals a mixture of monomers, dimers, and tetramers Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.2.5 ALLase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.5 55
-
 Mn2+-activited enzyme Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.5 50
-
 30 min, comnplete loss of activity Escherichia coli