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Literature summary extracted from

  • Moracci, M.; Capalbo, L.; Ciaramella, M.; Rossi, M.
    Identification of two glutamic acid residues essential for catalysis in the beta-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus (1996), Protein Eng., 9, 1191-1195.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B26 E206Q mutant shows 10- and 60-fold reduced activities on aryl-galacto and aryl-glucosides, respectively, when compared with the wild type. Significant decrease in Km-value with 4-nitrophenyl beta-D-glucoside or 2-nitrophenyl beta-D-glucoside. The residual activity of the mutant loses the typical pH dependence shown by the wild type. These data suggest that Glu206 acts as the general acid/base catalyst in the hydrolysis reaction Saccharolobus solfataricus
3.2.1.B26 E206Q mutant shows 10- and 60-fold reduced activities on aryl-galacto and aryl-glucosides, respectively, when compared with the wild type. Significant decrease in Km-value with 4-nitrophenyl beta-D-glucoside or 2-nitrophenyl betaD-glucoside. The residual activity of the mutant loses the typical pH dependence shown by the wild type. These data suggest that Glu206 acts as the general acid/base catalyst in the hydrolysis reaction Saccharolobus solfataricus
3.2.1.B26 E387Q mutant shows no detectable activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.03
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 0.03
-
 2-Nitrophenyl beta-D-glucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 0.09
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 0.3
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.45
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.95
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.01
-
 2-Nitrophenyl beta-D-glucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.17
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.53
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 4.79
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-
3.2.1.B26 Saccharolobus solfataricus MT4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B26 purification of mutant proteins E206Q and E387Q to homogeneity using the Schistosoma japonicum glutathione S-transferase fusion system Saccharolobus solfataricus
3.2.1.B26 purification of mutant proteins E206Q and E387Q to homogeneity using the Schistosoma japonicum glutathione-S-transferase fusion system Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus MT4 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 2-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 2-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus MT4 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus MT4 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus MT4 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus MT4 4-nitrophenol + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 Sbetagly
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 65
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 4.4
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 4.5
-
 2-Nitrophenyl beta-D-glucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 31
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 34
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 35
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 65°C, mutant enzyme E206Q Saccharolobus solfataricus
3.2.1.B26 240
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 252
-
 2-Nitrophenyl beta-D-glucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 263
-
 4-nitrophenyl beta-D-fucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 275
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 295
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 6.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B26 5.7 8 pH 5.6: about 45% of maximal activity, pH 8.0: about 55% of maximal activity Saccharolobus solfataricus