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Literature summary extracted from
- Identification and characterisation of a novel acylpeptide hydrolase from Sulfolobus solfataricus: structural and functional insights (2012), PLoS One, 7, e37921.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.1 | Guanidine-HCl | 1 M, complete loss of activity, after removal of guanidine-HCl the enzyme recovers 25% of its activity | Saccharolobus solfataricus |  |
| 3.4.19.1 | phosphatidylethanolamine-binding protein inhibitor SsCEI | - |
Saccharolobus solfataricus | |
| 3.4.19.1 | Sulfolobus solfataricus chymotrypsin-elastase inhibitor | specific inhibition | Saccharolobus solfataricus | |
| 3.4.19.1 | Urea | 6 M, about 70% loss of activity, regains activity at almost all urea concentrations upon removal of the denaturinf agent | Saccharolobus solfataricus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.04 | - |
N-acetyl-L-Phe-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 0.6 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 0.6 | - |
N-acetyl-L-Leu-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 0.8 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 0.8 | - |
N-acetyl-L-Ala-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 4 | - |
N-acetyl-L-phenylalanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.19.1 | cytosol | - |
Saccharolobus solfataricus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 64000 | - |
x * 64000, SDS-PAGE | Saccharolobus solfataricus |
| 3.4.19.1 | 64000 | - |
3 * 64000, SDS-PAGE | Saccharolobus solfataricus |
| 3.4.19.1 | 67000 | - |
3 * 67000, calculated from sequence | Saccharolobus solfataricus |
| 3.4.19.1 | 67000 | - |
x * 67000, calculated from amino acid sequence | Saccharolobus solfataricus |
| 3.4.19.1 | 192000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | guanidine-HCl | 1 M, complete loss of activity, after removal of guanidine-HCl the enzyme recovers 25% of its activity | Saccharolobus solfataricus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | Saccharolobus solfataricus | Q97VD6 | - |
- |
| 3.4.19.1 | Saccharolobus solfataricus P2 | Q97VD6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.1 | - |
Saccharolobus solfataricus |
| 3.4.19.1 | Q Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Mono S column chromatography | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 0.0007 | - |
cellular extract, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C | Saccharolobus solfataricus |
| 3.4.19.1 | 4.8 | - |
pH 7.5, 90°C | Saccharolobus solfataricus |
| 3.4.19.1 | 4.8 | - |
after 6857fold purification, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.1 | additional information | no activity with L-leucyl 4-nitroanilide, L-alanyl 4-nitroanilide or L-phenylalanyl 4-nitroanilide. The enzyme also shows endopeptidase activity | Saccharolobus solfataricus | ? | - |
? | |
| 3.4.19.1 | additional information | no activity with L-Leu-4-nitroanilide, L-Ala-4-nitroanilide, or L-Phe-4-nitroanilide, succinyl-AAPF-4-nitroanilide, and succinyl-AAVA-4-nitroanilide | Saccharolobus solfataricus | ? | - |
? | |
| 3.4.19.1 | additional information | no activity with L-leucyl 4-nitroanilide, L-alanyl 4-nitroanilide or L-phenylalanyl 4-nitroanilide. The enzyme also shows endopeptidase activity | Saccharolobus solfataricus P2 | ? | - |
? | |
| 3.4.19.1 | additional information | no activity with L-Leu-4-nitroanilide, L-Ala-4-nitroanilide, or L-Phe-4-nitroanilide, succinyl-AAPF-4-nitroanilide, and succinyl-AAVA-4-nitroanilide | Saccharolobus solfataricus P2 | ? | - |
? | |
| 3.4.19.1 | N-acetyl-L-Ala-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-Ala + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-alanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-alanine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-alanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus P2 | N-acetyl-L-alanine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-Leu-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-Leu + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-leucyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-leucine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-leucyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus P2 | N-acetyl-L-leucine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-Phe-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-Phe + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-phenylalanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-phenylalanine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | N-acetyl-L-phenylalanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus P2 | N-acetyl-L-phenylalanine + 4-nitroaniline | - |
? | |
| 3.4.19.1 | succinyl-AAA-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | succinyl-AAA + 4-nitroaniline | - |
? | |
| 3.4.19.1 | succinyl-GGF-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | succinyl-GGF + 4-nitroaniline | - |
? | |
| 3.4.19.1 | Z-GGL-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | Z-GGL + 4-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | homotrimer | x * 64000, SDS-PAGE | Saccharolobus solfataricus |
| 3.4.19.1 | homotrimer | 3 * 64000, SDS-PAGE | Saccharolobus solfataricus |
| 3.4.19.1 | homotrimer | 3 * 67000, calculated from sequence | Saccharolobus solfataricus |
| 3.4.19.1 | homotrimer | x * 67000, calculated from amino acid sequence | Saccharolobus solfataricus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | Acylaminoacyl-peptidase | - |
Saccharolobus solfataricus |
| 3.4.19.1 | acylpeptide hydrolase | - |
Saccharolobus solfataricus |
| 3.4.19.1 | APEH-3 | - |
Saccharolobus solfataricus |
| 3.4.19.1 | APEH-3Ss | - |
Saccharolobus solfataricus |
| 3.4.19.1 | SSO2693 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 90 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 60 | 90 | more than 50% activity between 60 and 90°C | Saccharolobus solfataricus |
| 3.4.19.1 | 80 | 95 | 80°C: about 65% of maximal activity, 95°C: about 50% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 70 | - |
24 h, 70% loss of activity. Slight increase its relative activity during the first 5 hours of incubation | Saccharolobus solfataricus |
| 3.4.19.1 | 70 | 90 | the enzyme retains 30% activity following the incubation for 24 h at 70°C while the residual activity of the enzyme is halved after 4 h incubation at 90°C | Saccharolobus solfataricus |
| 3.4.19.1 | 90 | - |
4 h, 50% loss of activity | Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 2 | - |
N-acetyl-L-Phe-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 8 | - |
N-acetyl-L-phenylalanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 14 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 17.5 | - |
N-acetyl-L-Ala-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 18 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 18 | - |
N-acetyl-L-Leu-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 7.5 | - |
- |
Saccharolobus solfataricus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 5 | 9 | more than 50% activity between pH 5.0 and 9.0 | Saccharolobus solfataricus |
| 3.4.19.1 | 7.5 | 8 | pH 7.5: about 55% of maximal activity, pH 8.0: about 75% of maximal activity | Saccharolobus solfataricus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.008 | - |
phosphatidylethanolamine-binding protein inhibitor SsCEI | pH 7.5, 90°C, substrate: acetyl-Leu-4-nitroanilide | Saccharolobus solfataricus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.4.19.1 | Saccharolobus solfataricus | expression of apeh-3 is down-regulated as an immediate response to stress | down |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.1 | 0.2 | - |
succinyl-AAA-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 0.7 | - |
Z-GGL-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 1.3 | - |
succinyl-GGF-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 2 | - |
N-acetyl-L-phenylalanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 2 | - |
N-acetyl-L-Phe-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 17.5 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 17.5 | - |
N-acetyl-L-Ala-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 32.7 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 90°C | Saccharolobus solfataricus | |
| 3.4.19.1 | 32.7 | - |
N-acetyl-L-Leu-4-nitroanilide | in 25 mM Tris-HCl buffer pH 7.5, at 90°C | Saccharolobus solfataricus | |
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