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Literature summary extracted from

  • Hole, K.; van Damme, P.; Dalva, M.; Aksnes, H.; Glomnes, N.; Varhaug, J.; Lillehaug, J.; Gevaert, K.; Arnesen, T.
    The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4 (2011), PLoS ONE, 6, e24713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.257 expressed in Escherichia coli BL21 Star(DE3) cells Homo sapiens
2.3.1.257 recombinant expression of hNaa40p in Escherichia coli strain BL21 StarTM (DE3), and of hNaa40p-V5 in HEK293 cells. Heterologous ectopically expression of hNaa40p in a Saccharomyces cerevisiae naa40-DELTA strain restores Nt-acetylation of yeast histone H4, but not H2A in vivo Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.257 cytosol
-
 Homo sapiens 5829
-
2.3.1.257 additional information endogenous hNaa40p localizes to both the cytoplasm and the nuclei Homo sapiens
-
-
2.3.1.257 nucleus
-
 Homo sapiens 5634
-
2.3.1.257 ribosome
-
 Homo sapiens 5840
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.257 acetyl-CoA + an N-terminal-L-seryl-[histone H2A] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
2.3.1.257 acetyl-CoA + an N-terminal-L-seryl-[histone H4] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?
2.3.1.257 acetyl-CoA + peptide Homo sapiens hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides CoA + Nalpha-acetylpeptide
-
 ?
2.3.1.257 additional information Homo sapiens hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.257 Homo sapiens Q86UY6
-
-
2.3.1.257 Homo sapiens Q86UY6 NatD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.257 HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens
2.3.1.257 recombinant hNaa40p-V5 from HEK293 cells by immunoprecipitation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.257 A-431 cell
-
 Homo sapiens
-
2.3.1.257 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.257 acetyl-CoA + an N-terminal-L-seryl-[histone H2A]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
2.3.1.257 acetyl-CoA + an N-terminal-L-seryl-[histone H4]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?
2.3.1.257 acetyl-CoA + histone H2A Saccharomyces cerevisiae histone H2A, 2SGGKGGKAGSAAKASQSR19 N-terminal sequence Homo sapiens CoA + Nalpha-acetyl-histone H2A
-
 ?
2.3.1.257 acetyl-CoA + histone H4 Saccharomyces cerevisiae histone H4, 2SGRGKGGKGLGKGGAKR18 N-terminal sequence. hNaa40p catalyzes H4 Nalpha-acetylation and not H4 lysine Nepsilon-acetylation, immunoprecipitated hNaa40p specifically Nt-acetylates H4 in vitro Homo sapiens CoA + Nalpha-acetyl-histone H4
-
 ?
2.3.1.257 acetyl-CoA + peptide
-
 Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
2.3.1.257 acetyl-CoA + peptide hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
2.3.1.257 additional information hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones Homo sapiens ?
-
 ?
2.3.1.257 additional information specificity determination using an in vitro proteome-derived peptide library Nt-acetylation assay, in vitro acetylation using synthetic oligopeptides as substrates, overview Homo sapiens ?
-
 ?
2.3.1.257 additional information the enzyme does not catalyze H4 lysine Nepsilon-acetylation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.257 hNaa40p/hNatD
-
 Homo sapiens
2.3.1.257 N-alpha-acetyltransferase 40
-
 Homo sapiens
2.3.1.257 Naa40p
-
 Homo sapiens
2.3.1.257 Naa40p catalytic subunit of NatD, also termed Nat4 Homo sapiens
2.3.1.257 NAT4
-
 Homo sapiens
2.3.1.257 NatD
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.257 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.257 8.3
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.257 acetyl-CoA
-
 Homo sapiens