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Literature summary extracted from

  • Liu, Y.L.; Chiang, Y.H.; Liu, G.Y.; Hung, H.C.
    Functional role of dimerization of human peptidylarginine deiminase 4 (PAD4) (2011), PLoS ONE, 6, e21314.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.15 D273A/R544A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.35 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.5/sec, quaternary structure: dimer, Kd: 0.68 microM Homo sapiens
3.5.3.15 D547A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 11.6/sec, quaternary structure: monomer/dimer, Kd: 6.4 microM Homo sapiens
3.5.3.15 D547E Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.72 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 8.9/sec, quaternary structure: monomer/dimer, Kd: 11.2 microM Homo sapiens
3.5.3.15 D547N Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.86 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 4.9 microM Homo sapiens
3.5.3.15 E281A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.45 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.1/sec, quaternary structure: dimer, Kd: 0.1 microM Homo sapiens
3.5.3.15 R8A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 9.3 microM Homo sapiens
3.5.3.15 R8A/D547A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.4 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.7/sec, quaternary structure: monomer/dimer, Kd: 3.9 microM Homo sapiens
3.5.3.15 R8E Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.06 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 7.3/sec, quaternary structure: monomer, Kd: 45.6 microM Homo sapiens
3.5.3.15 R8E/D547E Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.77 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 3.3/sec, quaternary structure: monomer, Kd: 24 microM Homo sapiens
3.5.3.15 R8H Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.3/sec, quaternary structure: dimer, Kd: 0.47 microM Homo sapiens
3.5.3.15 R8K Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.5 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.8/sec, quaternary structure: monomer/dimer, Kd: 10.2 microM Homo sapiens
3.5.3.15 R8L Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.3/sec, quaternary structure: monomer, Kd: 16.8 microM Homo sapiens
3.5.3.15 R8Q Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.6 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 15.7 microM Homo sapiens
3.5.3.15 Y237A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.9/sec, quaternary structure: dimer, Kd: 0.29 microM Homo sapiens
3.5.3.15 Y237A/E281A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.38 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.2/sec, quaternary structure: dimer, Kd: 0.1 microM Homo sapiens
3.5.3.15 Y435A Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.33 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.9/sec, quaternary structure: monomer, Kd: 30.3 microM Homo sapiens
3.5.3.15 Y435N Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.73 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 4/sec, quaternary structure: monomer, Kd: 33.8 microM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.15 0.31
-
 Nalpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.35
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D273A/R544A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.38
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A/E281A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.4
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A/D547A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.45
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant E281A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.47
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.47
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8H, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.5
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8K, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.6
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8Q , pH 7.4, 25°C Homo sapiens
3.5.3.15 0.72
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547E, pH 7.4, 25°C Homo sapiens
3.5.3.15 0.86
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547N, pH 7.4, 25°C Homo sapiens
3.5.3.15 1.06
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E, pH 7.4, 25°C Homo sapiens
3.5.3.15 1.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8L, pH 7.4, 25°C Homo sapiens
3.5.3.15 2.33
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.4, 25°C Homo sapiens
3.5.3.15 2.73
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435N, pH 7.4, 25°C Homo sapiens
3.5.3.15 2.77
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E/D547E, pH 7.4, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 Nalpha-benzoyl-L-arginine ethyl ester
-
 Homo sapiens Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.15 dimer dimerization of PAD4 is essential for full enzyme activity and calcium-binding cooperativity Homo sapiens
3.5.3.15 monomer monomeric PAD4 maintains 25-50% enzyme activity compared to the dimeric enzyme Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 PAD4
-
 Homo sapiens
3.5.3.15 peptidylarginine deiminase 4
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.15 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.15 3.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E/D547E, pH 7.4, 25°C Homo sapiens
3.5.3.15 4
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435N, pH 7.4, 25°C Homo sapiens
3.5.3.15 5.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8L, pH 7.4, 25°C Homo sapiens
3.5.3.15 5.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.4, 25°C Homo sapiens
3.5.3.15 7.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E, pH 7.4, 25°C Homo sapiens
3.5.3.15 8.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547E, pH 7.4, 25°C Homo sapiens
3.5.3.15 10.08
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8K, pH 7.4, 25°C Homo sapiens
3.5.3.15 10.7
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A/D547A, pH 7.4, 25°C Homo sapiens
3.5.3.15 11.6
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547A, pH 7.4, 25°C Homo sapiens
3.5.3.15 12.1
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant E281A, pH 7.4, 25°C Homo sapiens
3.5.3.15 12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547N, pH 7.4, 25°C Homo sapiens
3.5.3.15 12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A, pH 7.4, 25°C Homo sapiens
3.5.3.15 12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8Q , pH 7.4, 25°C Homo sapiens
3.5.3.15 13.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A/E281A, pH 7.4, 25°C Homo sapiens
3.5.3.15 13.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8H, pH 7.4, 25°C Homo sapiens
3.5.3.15 13.4
-
 Nalpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.4, 25°C Homo sapiens
3.5.3.15 13.5
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D273A/R544A, pH 7.4, 25°C Homo sapiens
3.5.3.15 13.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A, pH 7.4, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.15 7.4
-
 assay at Homo sapiens