Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Banerjee, A.; Rao, D.N.
    Functional analysis of an acid adaptive DNA adenine methyltransferase from Helicobacter pylori 26695 (2011), PLoS ONE, 6, e16810.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli BL21(DE3) pLysS cells Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 C54G the mutant has wild type activity Helicobacter pylori
2.1.1.72 Y107G inactive Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.72 S-adenosyl-L-methionine methylation reaction is inhibited above 0.0015 mM of S-adenosyl-L-methionine Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 0.00015
-
 DNA adenine at pH 5.5 and 37°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.72 Co2+ 3fold stimulation of activity at 1 mM Helicobacter pylori
2.1.1.72 Mg2+ 3.5fold stimulation of activity at 1 mM Helicobacter pylori
2.1.1.72 Mn2+ 5fold stimulation of activity at 1 mM Helicobacter pylori
2.1.1.72 additional information Ca2+, Zn2+, and Ni2+ ions do not affect the methylation activity of the enzyme Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 70600
-
 2 * 70600, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, MALDI-TOF mass spectrometry Helicobacter pylori
2.1.1.72 72000
-
 gel filtration, the enzyme occurs as both monomer and dimer in solution Helicobacter pylori
2.1.1.72 144000
-
 gel filtration, the enzyme occurs as both monomer and dimer in solution Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni2+-NTA column chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine duplex 2 DNA, 5'-GCAG-3' is the recognition site for the enzyme Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 homodimer 2 * 70600, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, MALDI-TOF mass spectrometry Helicobacter pylori
2.1.1.72 monomer 1 * 72000, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, gel filtration Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 HP0593 DNA-(N6-adenine)-methyltransferase
-
 Helicobacter pylori
2.1.1.72 HP0593 MTase
-
 Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.72 5.5
-
-
 Helicobacter pylori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.72 Helicobacter pylori isoelectric focusing
-
 5.8