Any feedback?
Literature summary extracted from
- Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica (2010), Planta Med., 76, 1921-1926.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.99 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of His-tagged HQT in Escherichia coli | Lonicera japonica |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.99 | 0.2936 | - |
caffeoyl-CoA | pH 7.5, 30°C, recombinant enzyme | Lonicera japonica |  |
| 2.3.1.99 | 0.7635 | - |
quinate | pH 7.5, 30°C, recombinant enzyme | Lonicera japonica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.99 | 48000 | - |
x * 48000, about, sequence calculation | Lonicera japonica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.99 | caffeoyl-CoA + quinate | Lonicera japonica | - |
CoA + chlorogenic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.99 | Lonicera japonica | D1L8A9 | - |
- |
| 2.3.1.99 | no activity in Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.99 | recombinant His-tagged HQT from Escherichia coli by nickel affinity chromatography | Lonicera japonica |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.3.1.99 | flower bud | high enzyme content | Lonicera japonica | - |
| 2.3.1.99 | leaf | - |
Lonicera japonica | - |
| 2.3.1.99 | stem | lower enzyme content | Lonicera japonica | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.99 | caffeoyl-CoA + quinate | - |
Lonicera japonica | CoA + chlorogenic acid | - |
? | |
| 2.3.1.99 | caffeoyl-CoA + quinate | - |
Lonicera japonica | CoA + chlorogenic acid | i.e. chlorogenate | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.1.99 | ? | x * 48000, about, sequence calculation | Lonicera japonica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.99 | HQT | - |
Lonicera japonica |
| 2.3.1.99 | hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase | - |
Lonicera japonica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.99 | 30 | - |
assay at | Lonicera japonica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.99 | 7.5 | - |
assay at | Lonicera japonica |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.3.1.99 | metabolism | HQT is involved in one of three possible pathways of chlorogenate biosynthesis, overview | Lonicera japonica |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
