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Literature summary extracted from

  • Matsushima, D.; Jenke-Kodama, H.; Sato, Y.; Fukunaga, Y.; Sumimoto, K.; Kuzuyama, T.; Matsunaga, S.; Okada, S.
    The single cellular green microalga Botryococcus braunii, race B possesses three distinct 1-deoxy-D-xylulose 5-phosphate synthases (2012), Plant Sci., 185-186, 309-320.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.7
-
 Botryococcus braunii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.7 0.47
-
 D-glyceraldehyde 3-phosphate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 0.72
-
 pyruvate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 0.75
-
 pyruvate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 0.87
-
 D-glyceraldehyde 3-phosphate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 1
-
 D-glyceraldehyde 3-phosphate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 1.8
-
 pyruvate pH 7.8, 32°C Botryococcus braunii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.7 plastid sequence contains a plastid targeting peptide Botryococcus braunii 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.7 Mg2+ required, maximal activity at 4-12 mM Botryococcus braunii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.7 72500
-
 x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein Botryococcus braunii
2.2.1.7 77400
-
 x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii
2.2.1.7 77400
-
 x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii
2.2.1.7 78500
-
 x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein Botryococcus braunii
2.2.1.7 82300
-
 x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii
2.2.1.7 83100
-
 x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.7 Botryococcus braunii H5ZXI0 isoform DXS-II
-
2.2.1.7 Botryococcus braunii H5ZXI1 isoform DXS-II
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.2.1.7 proteolytic modification sequence contains a plastid targeting peptide Botryococcus braunii

Storage Stability

EC Number Storage Stability Organism
2.2.1.7 -20°C, elution buffer containing 30% glycerol, storage for around 10 days with no significant loss of activity Botryococcus braunii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Botryococcus braunii 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.7 ? x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein Botryococcus braunii
2.2.1.7 ? x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii
2.2.1.7 ? x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein Botryococcus braunii

Synonyms

EC Number Synonyms Comment Organism
2.2.1.7 DXS-I
-
 Botryococcus braunii
2.2.1.7 DXS-II
-
 Botryococcus braunii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.7 32
-
-
 Botryococcus braunii
2.2.1.7 34
-
-
 Botryococcus braunii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.7 50
-
 75% of the activity at 34°C Botryococcus braunii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.7 35
-
 sharp decrease in activity above Botryococcus braunii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.7 1.9
-
 pyruvate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 2
-
 D-glyceraldehyde 3-phosphate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 2.4
-
 pyruvate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 5.6
-
 pyruvate pH 7.8, 32°C Botryococcus braunii
2.2.1.7 6
-
 D-glyceraldehyde 3-phosphate pH 7.8, 32°C Botryococcus braunii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.7 8.5 9
-
 Botryococcus braunii

Expression

EC Number Organism Comment Expression
2.2.1.7 Botryococcus braunii mRNA accumulation patterns over time are almost identical among the three DXS isoforms reaching the highest expression levels on day 6 after inoculation into fresh medium additional information