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Literature summary extracted from

  • Sue, M.; Ishihara, A.; Iwamura, H.
    Purification and characterization of a beta-glucosidase from rye (Secale cereale L.) seedlings (2000), Plant sci., 155, 67-74.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.182 castanospermine
-
 Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.182 0.616
-
 4-nitrophenyl beta-D-fucoside pH 5.5, 25°C Secale cereale
3.2.1.182 0.617
-
 DIMBOA-glucoside pH 5.5, 25°C Secale cereale
3.2.1.182 0.89
-
 2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside pH 5.5, 25°C Secale cereale
3.2.1.182 0.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 25°C Secale cereale
3.2.1.182 1.19
-
 DIBOA-glucoside pH 5.5, 25°C Secale cereale
3.2.1.182 2
-
 2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside pH 5.5, 25°C Secale cereale
3.2.1.182 3.17
-
 4-nitrophenyl beta-D-xyloside pH 5.5, 25°C Secale cereale

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.182 Ag+ 0.2 mM, complete inhibition Secale cereale
3.2.1.182 Cu2+ 0.5 mM, strong inhibition Secale cereale
3.2.1.182 Fe2+ 0.5 mM, inhibits activity Secale cereale
3.2.1.182 Mg2+ 0.5 mM, inhibits activity Secale cereale
3.2.1.182 Mn2+ 0.5 mM, inhibits activity Secale cereale
3.2.1.182 Zn2+ 0.5 mM, inhibits activity Secale cereale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.182 60000
-
 SDS-PAGE, monomer Secale cereale
3.2.1.182 300000
-
 native-PAGE, oligomer Secale cereale

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.182 Secale cereale
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.182 using isoelectric precipitation, anion exchange chromatography, and gel filtration Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.182 seedling
-
 Secale cereale
-
3.2.1.182 shoot isolated from 48-h-old rye shoots Secale cereale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.182 2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O
-
 Secale cereale 2-hydroxy-1,4-benzoxazin-3-one + beta-D-glucose
-
 ?
3.2.1.182 2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
-
 Secale cereale 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
 ?
3.2.1.182 4-nitrophenyl beta-D-fucoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.182 4-nitrophenyl beta-D-glucoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.182 4-nitrophenyl beta-D-xyloside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.182 DIBOA-glucoside + H2O
-
 Secale cereale DIBOA + beta-D-glucose
-
 ?
3.2.1.182 DIMBOA-glucoside + H2O
-
 Secale cereale DIMBOA + beta-D-glucose
-
 ?
3.2.1.182 esculin + H2O
-
 Secale cereale esculetin + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.182 oligomer native-PAGE Secale cereale

Synonyms

EC Number Synonyms Comment Organism
3.2.1.182 beta-glucosidase
-
 Secale cereale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.182 25
-
 optimal reaction at 25-30°C Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.182 5.5
-
-
 Secale cereale