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Literature summary extracted from

  • Onkokesung, N.; Gaquerel, E.; Kotkar, H.; Kaur, H.; Baldwin, I.; Galis, I.
    MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A: polyamine transferases in Nicotiana attenuata (2012), Plant Physiol., 158, 389-407.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.138 AT1 gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged AT1 in Escherichia coli strain BL21(DE3) Nicotiana attenuata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.138 0.024
-
 caffeoyl-CoA with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C Nicotiana attenuata
2.3.1.138 0.664
-
 putrescine with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C Nicotiana attenuata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.138 caffeoyl-CoA + putrescine Nicotiana attenuata
-
 CoA + N-caffeoylputrescine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.138 Nicotiana attenuata G9HTF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.138 recombinant C-terminally His6-tagged AT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Nicotiana attenuata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.138 leaf
-
 Nicotiana attenuata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.138 caffeoyl-CoA + putrescine
-
 Nicotiana attenuata CoA + N-caffeoylputrescine
-
 ?
2.3.1.138 caffeoyl-CoA + putrescine the enzyme is highly specific for putrescine and caffeoyl-CoA Nicotiana attenuata CoA + N-caffeoylputrescine
-
 ?
2.3.1.138 feruloyl-CoA + putrescine feruloyl-CoA gives 10% of the activity with caffeoyl-CoA Nicotiana attenuata CoA + N-feruloylputrescine
-
 ?
2.3.1.138 additional information substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA Nicotiana attenuata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.138 AT1
-
 Nicotiana attenuata
2.3.1.138 hydroxycinnamoyl-coenzyme A:putrescine acyltransferase
-
 Nicotiana attenuata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.138 30
-
 assay at Nicotiana attenuata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.138 7.5
-
 assay at Nicotiana attenuata

Expression

EC Number Organism Comment Expression
2.3.1.138 Nicotiana attenuata AT1 expression is controlled by the key transcription factor MYB8 additional information

General Information

EC Number General Information Comment Organism
2.3.1.138 malfunction deregulatory effects of transcription factor MYB8 silencing on AT1 control, altered metabolite profile, overview. Silencing of acyltransferase AT1 impairs polyamine accumulation Nicotiana attenuata
2.3.1.138 metabolism the enzyme AT1 is involved in a branch of jasmonic acid signaling that defends Nicotiana attenuata plants against herbivores: jasmonic acid, via key transcription factor MYB8's transcriptional control of AT1 and DH29 genes, controls the entire branch of polyamine biosynthesis, overview. AT1, DH29, and CV86 contribute to distinct, but interconnected, MYB8-controlled hydroxycinnamoylputrescine and hydroxycinnamoylspermidine pools Nicotiana attenuata
2.3.1.138 physiological function the enzyme is responsible for caffeoylputrescine biosynthesis in tobacco Nicotiana attenuata