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Literature summary extracted from

  • Yoon, K.; Han, D.; Li, Y.; Sommerfeld, M.; Hu, Q.
    Phospholipid:diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation while synthesizing triacylglycerol in the unicellular green microalga Chlamydomonas reinhardtii (2012), Plant Cell, 24, 3708-3724.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.158 energy production the strong lipase activity of PDAT with broad substrate specificity might be a potential biocatalyst for industrial lipid hydrolysis and conversion, particularly for biofuel production Chlamydomonas reinhardtii
2.3.1.158 industry the strong lipase activity of PDAT with broad substrate specificity might be a potential biocatalyst for industrial lipid hydrolysis and conversion, particularly for biofuel production Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.158 gene encoding PDAT, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of full-length enzyme and of truncated PDAT lacking the transmembrane domain in Pichia pastoris Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.158 membrane
-
 Chlamydomonas reinhardtii 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.158 95000
-
 x * 104600, sequence calculation, x * 120000, recombinant full-length PDAT, SDS-PAGE, x * 95000, recombinant truncated PDAT lacking the transmembrane domain, SDS-PAGE Chlamydomonas reinhardtii
2.3.1.158 104600
-
 x * 104600, sequence calculation, x * 120000, recombinant full-length PDAT, SDS-PAGE, x * 95000, recombinant truncated PDAT lacking the transmembrane domain, SDS-PAGE Chlamydomonas reinhardtii
2.3.1.158 120000
-
 x * 104600, sequence calculation, x * 120000, recombinant full-length PDAT, SDS-PAGE, x * 95000, recombinant truncated PDAT lacking the transmembrane domain, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol Chlamydomonas reinhardtii PDAT uses membrane lipids (e.g., phospholipids and glycolipids) as the substrates for the acyl transfer reaction or hydrolysis in vivo lysophospholipid + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.158 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.158 recombinant full-length enzyme and truncated PDAT lacking the transmembrane domain from Pichia pastoris by affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.158 additional information PDAT shows broad substrate specificity, overview Chlamydomonas reinhardtii ?
-
 ?
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol PDAT uses membrane lipids (e.g., phospholipids and glycolipids) as the substrates for the acyl transfer reaction or hydrolysis in vivo Chlamydomonas reinhardtii lysophospholipid + triacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.158 ? x * 104600, sequence calculation, x * 120000, recombinant full-length PDAT, SDS-PAGE, x * 95000, recombinant truncated PDAT lacking the transmembrane domain, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
2.3.1.158 PDAT
-
 Chlamydomonas reinhardtii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.158 Chlamydomonas reinhardtii sequence calculation
-
 5.96

Expression

EC Number Organism Comment Expression
2.3.1.158 Chlamydomonas reinhardtii Cr-PDAT is transiently upregulated in response to N deprivation. The protein expression achieves the maximum level at 3 h after the onset of N depletion from the culture medium and then gradually decreases during the following 48 h up

General Information

EC Number General Information Comment Organism
2.3.1.158 evolution PDAT belongs to the LCAT-like family Chlamydomonas reinhardtii
2.3.1.158 malfunction artificial microRNA silencing of PDAT alters the membrane lipid composition, reducing the maximum specific growth rate Chlamydomonas reinhardtii
2.3.1.158 physiological function phospholipid:diacylglycerol acyltransferase in the green microalga Chlamydomonas reinhardtii catalyzes triacylglycerol synthesis via two pathways: transacylation of diacylglycerol with acyl groups from phospholipids and galactolipids and diacylglycerol:diacylglycerol transacylation. PDAT-mediated membrane lipid turnover and triacylglycerol synthesis is essential for vigorous growth under favorable culture conditions and for membrane lipid degradation with concomitant production of triacylglycerol for survival under stress. PDAT also possesses acyl hydrolase activities using triacylglycerols, phospholipids, galactolipids, and cholesteryl esters as substrates Chlamydomonas reinhardtii