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Literature summary extracted from

  • Galvez-Valdivieso, G.; Cardenosa, R.; Vera, J.M.; Pineda, M.; Aguilar, M.
    gamma-Tocopherol methyltransferase from the green alga Chlamydomonas reinhardtii: functional characterization and expression analysis (2011), Physiol. Plant., 143, 316-328.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.95 dithiothreitol
-
 Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.95 expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.95 0.0018
-
 gamma-tocopherol in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii
2.1.1.95 0.0028
-
 delta-tocopherol in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii
2.1.1.95 0.0087
-
 S-adenosyl-L-methionine in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.95 33971
-
 1 * 33971, calculated from amino acid sequence Chlamydomonas reinhardtii
2.1.1.95 37000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.95 Chlamydomonas reinhardtii Q5CB51
-
-
2.1.1.95 Chlamydomonas reinhardtii 6145c Q5CB51
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.95 glutathione-agarose column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.95 S-adenosyl-L-methionine + delta-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + beta-tocopherol
-
 ?
2.1.1.95 S-adenosyl-L-methionine + delta-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii 6145c S-adenosyl-L-homocysteine + beta-tocopherol
-
 ?
2.1.1.95 S-adenosyl-L-methionine + gamma-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?
2.1.1.95 S-adenosyl-L-methionine + gamma-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii 6145c S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.95 monomer 1 * 37000, SDS-PAGE Chlamydomonas reinhardtii
2.1.1.95 monomer 1 * 33971, calculated from amino acid sequence Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
2.1.1.95 gamma-TMT
-
 Chlamydomonas reinhardtii
2.1.1.95 gamma-tocopherol methyltransferase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.95 40
-
 around 40°C Chlamydomonas reinhardtii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.95 30 50 about 60% activity at 30°C, about 55% activity at 50°C Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.95 4 45 the enzyme is stable at 4°C, shows about 80% activity after 10 min at 30-35°C, about 60% activity after 10 min at 40°C, and is completely inactivated after 10 min at 45°C Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.95 7.5
-
-
 Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.95 6 9 about 60% activity at pH 6.0, about 50% activity at pH 9.0 Chlamydomonas reinhardtii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.95 6 10 the enzyme is stable above pH 6.0 (more than 90% activity), even at alkaline pH 10.0 Chlamydomonas reinhardtii

Expression

EC Number Organism Comment Expression
2.1.1.95 Chlamydomonas reinhardtii enzyme expression is downregulated (to 40%) by low temperature (10°C) down
2.1.1.95 Chlamydomonas reinhardtii enzyme expression is strongly (about 80%) upregulated by high light up