Literature summary extracted from
Bastos, M.; Coutinho, B.; Coelho, M.
Lysostaphin: A staphylococcal bacteriolysin with potential clinical applications (2010), Pharmaceuticals, 3, 1139-1161.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.24.75 |
medicine |
lysostaphin is an antistaphylococcal agent. The enzyme is efficacious in the treatment of bovine mastitis caused by staphylococci |
Staphylococcus simulans |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.75 |
expressed in Escherichia coli |
Staphylococcus simulans |
General Stability
EC Number |
General Stability |
Organism |
---|
3.4.24.75 |
lysostaphin is relatively stable when conjugated with polyethylene glycol and it maintains its activity in human serum |
Staphylococcus simulans |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.24.75 |
Zn2+ |
contains zinc |
Staphylococcus simulans |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.24.75 |
27000 |
- |
1 * 27000, calculated from amino acid sequence |
Staphylococcus simulans |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.4.24.75 |
additional information |
Staphylococcus simulans |
lysostaphin exhibits a high degree of antistaphylococcal bacteriolytic activity, being inactive against bacteria of all other genera. The cell-wall degrading activity of lysostaphin is primarily due to a glycylglycine endopeptidase activity. Staphylococcus simulans biovar staphylolyticus peptidoglycan is resistant to the hydrolytic activity of lysostaphin, since the cells produce a resistance factor that causes the incorporation of Ser residues into the third and fifth positions of the cell wall cross bridges |
? |
- |
? |
|
3.4.24.75 |
Peptidoglycan + H2O |
Staphylococcus simulans |
cleaves Gly-Gly bonds in peptidoglycans |
Fragments of peptidoglycan |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.75 |
Staphylococcus simulans |
P10547 |
Staphylococcus simulans biovar staphylolyticus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.24.75 |
additional information |
lysostaphin exhibits a high degree of antistaphylococcal bacteriolytic activity, being inactive against bacteria of all other genera. The cell-wall degrading activity of lysostaphin is primarily due to a glycylglycine endopeptidase activity. Staphylococcus simulans biovar staphylolyticus peptidoglycan is resistant to the hydrolytic activity of lysostaphin, since the cells produce a resistance factor that causes the incorporation of Ser residues into the third and fifth positions of the cell wall cross bridges |
Staphylococcus simulans |
? |
- |
? |
|
3.4.24.75 |
Peptidoglycan + H2O |
cleaves Gly-Gly bonds in peptidoglycans |
Staphylococcus simulans |
Fragments of peptidoglycan |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.24.75 |
monomer |
1 * 27000, calculated from amino acid sequence |
Staphylococcus simulans |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.24.75 |
7.5 |
- |
- |
Staphylococcus simulans |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.4.24.75 |
Staphylococcus simulans |
calculated from amino acid sequence |
- |
9.5 |