Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hu, J.; Guo, L.; Wu, K.; Liu, B.; Lang, S.; Huang, L.
    Template-dependent polymerization across discontinuous templates by the heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus (2012), Nucleic Acids Res., 40, 3470-3483.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.B16 dNTP + n dNTP Saccharolobus solfataricus
-
 dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 dNTP + n dNTP Saccharolobus solfataricus P2
-
 dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP Saccharolobus solfataricus
-
 N(pN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP Saccharolobus solfataricus P2
-
 N(pN)n + n diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Saccharolobus solfataricus Q9UWW1 and Q97Z83 Q9UWW1: small subunit, Q97Z83: large subunit
-
2.7.7.B16 Saccharolobus solfataricus P2 Q9UWW1 and Q97Z83 Q9UWW1: small subunit, Q97Z83: large subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B16 ATP + n ATP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus A(pA)n + n diphosphate
-
 ?
2.7.7.B16 ATP + n ATP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 A(pA)n + n diphosphate
-
 ?
2.7.7.B16 dNTP + n dNTP
-
 Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 dNTP + n dNTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 dNTP + n dNTP
-
 Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 dNTP + n dNTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
2.7.7.B16 GTP + n GTP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus G(pG)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP
-
 Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP
-
 Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B16 SsoPriSL
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.B16 55 70 polymerization reactions on C35 or C34ddC with rGTP. Both the size and the quantity of the products on C35 increase, while synthesis on C34ddC drastically decreases, when the reaction temperature is raised from 55°C to 70°C Saccharolobus solfataricus