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Literature summary extracted from

  • Desmolaize, B.; Fabret, C.; Bregeon, D.; Rose, S.; Grosjean, H.; Douthwaite, S.
    A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA (2011), Nucleic Acids Res., 39, 9368-9375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.189 gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain Bacillus subtilis
2.1.1.190 gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.189 additional information Bacillus subtilis methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation ?
-
 ?
2.1.1.189 additional information Bacillus subtilis 168 methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation ?
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA Bacillus subtilis
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA Bacillus subtilis 168
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.190 additional information Bacillus subtilis methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation ?
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA Bacillus subtilis
-
 S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.189 Bacillus subtilis O31503 YefA; gene yefA
-
2.1.1.189 Bacillus subtilis 168 O31503 YefA; gene yefA
-
2.1.1.190 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.189 additional information methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation Bacillus subtilis ?
-
 ?
2.1.1.189 additional information methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation Bacillus subtilis 168 ?
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Bacillus subtilis S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Bacillus subtilis 168 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.190 additional information methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation Bacillus subtilis ?
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA
-
 Bacillus subtilis S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.189 RlmCD
-
 Bacillus subtilis
2.1.1.189 YefA
-
 Bacillus subtilis
2.1.1.190 RlmCD
-
 Bacillus subtilis
2.1.1.190 YefA
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.189 S-adenosyl-L-methionine
-
 Bacillus subtilis
2.1.1.190 S-adenosyl-L-methionine
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.1.1.189 evolution YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD Bacillus subtilis
2.1.1.190 evolution YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD Bacillus subtilis