BRENDA - Enzyme Database

Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins

Guranowski, A.; Wojdyla, A.; Zimny, J.; Wypijewska, A.; Kowalska, J.; Lukaszewicz, M.; Jemielity, J.; Darzynkiewicz, E.; Jagiello, A.; Bieganowski, P.; New J. Chem. 34, 888-893 (2010)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.29
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Arabidopsis thaliana
3.6.1.29
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Homo sapiens
3.6.1.29
0.015
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Trypanosoma brucei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.29
Arabidopsis thaliana
-
-
-
3.6.1.29
Homo sapiens
-
-
-
3.6.1.29
Trypanosoma brucei
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
100% initial velocity of degradationin 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
64% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
96% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
19% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
25% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
97% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
100% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
48% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
65% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
1% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
100% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
8% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
1% initial velocities of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
100% initial velocities of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
8% initial velocities of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl) triphosphate + H2O
27% initial velocity of degradation
720558
Arabidopsis thaliana
ADP + AMP
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl) triphosphate + H2O
58% initial velocity of degradation
720558
Homo sapiens
ADP + AMP
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl)triphosphate + H2O
72% initial velocity of degradation
720558
Trypanosoma brucei
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
27% initial velocities of degradation
720558
Arabidopsis thaliana
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
58% initial velocities of degradation
720558
Homo sapiens
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
72% initial velocities of degradation
720558
Trypanosoma brucei
ADP + AMP
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.29
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Arabidopsis thaliana
3.6.1.29
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Homo sapiens
3.6.1.29
0.015
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
100% initial velocity of degradationin 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
64% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
96% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
19% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
25% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
97% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
100% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
48% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
65% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
1% initial velocity of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
100% initial velocity of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
8% initial velocity of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
1% initial velocities of degradation
720558
Trypanosoma brucei
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
100% initial velocities of degradation
720558
Arabidopsis thaliana
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
8% initial velocities of degradation
720558
Homo sapiens
?
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl) triphosphate + H2O
27% initial velocity of degradation
720558
Arabidopsis thaliana
ADP + AMP
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl) triphosphate + H2O
58% initial velocity of degradation
720558
Homo sapiens
ADP + AMP
-
-
-
?
3.6.1.29
P1,P3-bis(5'-adenosyl)triphosphate + H2O
72% initial velocity of degradation
720558
Trypanosoma brucei
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
27% initial velocities of degradation
720558
Arabidopsis thaliana
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
58% initial velocities of degradation
720558
Homo sapiens
ADP + AMP
-
-
-
?
3.6.1.29
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
72% initial velocities of degradation
720558
Trypanosoma brucei
ADP + AMP
-
-
-
?