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Literature summary extracted from

  • Wu, B.; Zhang, B.; Feng, X.; Rubens, J.; Huang, R.; Hicks, L.; Pakrasi, H.; Tang, Y.
    Alternative isoleucine synthesis pathway in cyanobacterial species (2010), Microbiology, 156, 596-602.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.21 synthesis the autrotrophic micro-organism may be engineered for robust butanol and propanol production from 2-ketobutyrate, which is an intermediate in the isoleucine biosynthesis pathway Crocosphaera subtropica ATCC 51142

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O Crocosphaera subtropica ATCC 51142
-
 CoA + (2R)-2-hydroxy-2-methylbutanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.21 Crocosphaera subtropica ATCC 51142
-
 an aerobic unicellular marine cyanobacterium, gene cimA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O
-
 Crocosphaera subtropica ATCC 51142 CoA + (2R)-2-hydroxy-2-methylbutanedioate
-
 ?
2.3.3.21 acetyl-CoA + pyruvate + H2O
-
 Crocosphaera subtropica ATCC 51142 CoA + (2R)-2-hydroxy-2-methylbutanedioate i.e. (-)-citramalate ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.21 CimA
-
 Crocosphaera subtropica ATCC 51142
2.3.3.21 citramalate synthase
-
 Crocosphaera subtropica ATCC 51142

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.21 7.5
-
 assay at Crocosphaera subtropica ATCC 51142

General Information

EC Number General Information Comment Organism
2.3.3.21 metabolism citramalate synthase acts in the alternative isoleucine synthesis pathway present in cyanobacteria, such as Cyanothece and Synechococcus. The pathway has been detected first in mostly anaerobic bacteria or archaea Crocosphaera subtropica ATCC 51142
2.3.3.21 physiological function synthesis of isoleucine from citramalate via condensation of acetyl-CoA and pyruvate catalysed by citramalate synthase, CimA Crocosphaera subtropica ATCC 51142