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Literature summary extracted from

  • Huang, H.; Berg, S.; Spencer, J.S.; Vereecke, D.; DHaeze, W.; Holsters, M.; McNeil, M.R.
    Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis (2008), Microbiology, 154, 736-743.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.45 expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis
2.4.2.45 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum
2.4.2.45 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.45 A66F inactive Mycobacterium tuberculosis
2.4.2.45 D74A inactive Mycobacterium tuberculosis
2.4.2.45 D77A inactive Mycobacterium tuberculosis
2.4.2.45 D77E 15% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 D81A inactive Mycobacterium tuberculosis
2.4.2.45 E195A 17% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 F59A 94% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 F62A 72% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 H84L 15% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 N29A 18% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 N73A inactive Mycobacterium tuberculosis
2.4.2.45 N73Q 15% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 R192A inactive Mycobacterium tuberculosis
2.4.2.45 R201A 1% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 R22L 10% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.4.2.45 Y70A inactive Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.45 plasma membrane
-
 Mycolicibacterium smegmatis 5886
-
2.4.2.45 plasma membrane
-
 Corynebacterium glutamicum 5886
-
2.4.2.45 plasma membrane
-
 Mycobacterium tuberculosis 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.45 Corynebacterium glutamicum
-
-
-
2.4.2.45 Mycobacterium tuberculosis P9WFR5
-
-
2.4.2.45 Mycolicibacterium smegmatis
-
-
-
2.4.2.45 no activity in Azorhizobium caulinodans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Corynebacterium glutamicum trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.45 5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate
-
 Mycolicibacterium smegmatis
2.4.2.45 5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate
-
 Corynebacterium glutamicum
2.4.2.45 5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate
-
 Mycobacterium tuberculosis
2.4.2.45 5-phosphoribosyltransferase
-
 Mycolicibacterium smegmatis
2.4.2.45 5-phosphoribosyltransferase
-
 Corynebacterium glutamicum
2.4.2.45 5-phosphoribosyltransferase
-
 Mycobacterium tuberculosis
2.4.2.45 cg3189
-
 Corynebacterium glutamicum
2.4.2.45 DPPR synthase
-
 Mycolicibacterium smegmatis
2.4.2.45 DPPR synthase
-
 Corynebacterium glutamicum
2.4.2.45 DPPR synthase
-
 Mycobacterium tuberculosis
2.4.2.45 MSMEG 6401
-
 Mycolicibacterium smegmatis
2.4.2.45 Rv3806c
-
 Mycobacterium tuberculosis