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Literature summary extracted from

  • Lee, S.J.; Kim, d.o..J.; Kim, H.S.; Lee, B.I.; Yoon, H.J.; Yoon, J.Y.; Kim, K.H.; Jang, J.Y.; Im, H.N.; An, D.R.; Song, J.S.; Kim, H.J.; Suh, S.W.
    Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily (2011), J. Struct. Biol., 175, 329-338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.7
-
 Pseudomonas aeruginosa
3.5.3.17 expressed in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.7 crystal structures of Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) are determined. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops Pseudomonas aeruginosa
3.5.3.17 crystal structures are determined of Mn2+-bound GpuA (methylated), and 1,6-diaminohexane-bound GpuA (unmethylated) Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.7 M161Y mutant showed no detectable enzymatic activity against guanidium compounds Pseudomonas aeruginosa
3.5.3.17 Y157M Km for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly increased compared to wild-type, kcat for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly decreased Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 10.7
-
 4-Guanidinobutyrate pH 7.9, 30°C, wild-type Pseudomonas aeruginosa
3.5.3.17 12.8
-
 3-guanidinopropionate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 37.3
-
 4-Guanidinobutyrate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 108
-
 3-guanidinopropionate pH 7.9, 30°C, mutant Y157M unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 285
-
 4-Guanidinobutyrate pH 7.9, 30°C, mutant Y157M unmethylated GpuA Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Pseudomonas aeruginosa
-
-
-
3.5.3.17 Pseudomonas aeruginosa Q9I6K2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O enzyme shows no activity towards 3-guanidinopropionate Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?
3.5.3.17 3-guanidinopropionate + H2O
-
 Pseudomonas aeruginosa beta-alanine + urea
-
 ?
3.5.3.17 4-Guanidinobutyrate + H2O
-
 Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.7 GbuA
-
 Pseudomonas aeruginosa
3.5.3.7 guanidinobutyrase
-
 Pseudomonas aeruginosa
3.5.3.17 GpuA
-
 Pseudomonas aeruginosa
3.5.3.17 guanidinopropionase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.7 30
-
 assay at Pseudomonas aeruginosa
3.5.3.17 30
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.7 94.7
-
 4-Guanidinobutyrate pH 7.9, 30°C, wild-type Pseudomonas aeruginosa
3.5.3.17 2 8 4-Guanidinobutyrate pH 7.9, 30°C, mutant Y157M unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 48.8
-
 3-guanidinopropionate pH 7.9, 30°C, mutant Y157M unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 57.6
-
 4-Guanidinobutyrate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 106
-
 3-guanidinopropionate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 7.9
-
 assay at Pseudomonas aeruginosa
3.5.3.17 7.9
-
 assay at Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.17 1.54
-
 4-Guanidinobutyrate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa
3.5.3.17 8.28
-
 3-guanidinopropionate pH 7.9, 30°C, wild-type unmethylated GpuA Pseudomonas aeruginosa