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Literature summary extracted from

  • Nango, E.; Eguchi, T.; Kakinuma, K.
    Active site mapping of 2-deoxy-scyllo-inosose synthase, the key starter enzyme for the biosynthesis of 2-deoxystreptamine. Mechanism-based inhibition and identification of lysine-141 as the entrapped nucleophile (2004), J. Org. Chem., 69, 593-600.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.124 gene btrC, expression in Escherichia coli Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.124 DL-carbaglucose 6-phosphate a mechanism-based irreversible inhibitor, synthesis, overview. The alpha,beta-unsaturated intermediate traps a specific nucleophilic group in the active site through the Michael-type 1,4-addition. The covalently modified amino acid residue is Lys141 Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.124 Co2+ required Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.124 40608
-
 x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry Niallia circulans
4.2.3.124 40768
-
 x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate Niallia circulans
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate Niallia circulans SANK72073
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.124 Niallia circulans Q9S5E2 gene btrC
-
4.2.3.124 Niallia circulans SANK72073 Q9S5E2 gene btrC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.124 recombinant BtrC from Escherichia coli by gel filtration and hydrophobic interaction chromatography Niallia circulans

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.124 D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate reaction mechanism, overview Niallia circulans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate
-
 Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate
-
 Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.124 ? x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
4.2.3.124 btrC
-
 Niallia circulans
4.2.3.124 DOIS
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.124 46
-
 assay at Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.124 7.7
-
 assay at Niallia circulans

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.124 NAD+ required Niallia circulans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.3.124 0.224
-
 DL-carbaglucose 6-phosphate pH 7.7, 46°C Niallia circulans