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Literature summary extracted from

  • Moreno-Vargas, L.; Correa-Basurto, J.; Maroun, R.C.; Fernandez, F.J.
    Homology modeling of the structure of acyl coA:isopenicillin N-acyltransferase (IAT) from Penicillium chrysogenum. IAT interaction studies with isopenicillin-N, combining molecular dynamics simulations and docking (2012), J. Mol. Model., 18, 1189-1205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.164 gene penDE Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.164 40000
-
 pro-enzyme Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
 CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum P15802 gene penDE
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.164 proteolytic modification the proenzyme is processed autocatalytically to generate two polypeptides forming alpha-subunit and beta-subunit Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O IAT substitutes the alpha-aminoadipic acid side chain of isopenicillin N by a phenylacetic acid phenolate group from phenylacetyl-CoA Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.164 dimer three-dimensional structure determined by homology modeling and molecular docking in different snapshots, and refined by molecular dynamic simulations, detailed overview. The gene for IAT, penDE, encodes a 357 residue proenzyme with a molecular weight of 40 kDa, which is processed autocatalytically to generate two polypeptides of 102 residues as alpha-subunit and 255 residues as beta-subunit Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.164 acyl coA:isopenicillin N-acyltransferase
-
 Penicillium chrysogenum
2.3.1.164 IAT
-
 Penicillium chrysogenum

General Information

EC Number General Information Comment Organism
2.3.1.164 metabolism in the last step of penicillin biosynthesis, acyl-CoA:isopenicillin N acyltransferase catalyzes the conversion of isopenicillin N to penicillin G Penicillium chrysogenum
2.3.1.164 additional information substrate binding structure determined by homology modeling and molecular docking in different snapshots, and refined by molecular dynamic simulations. A site located in the region generated by beta1, beta2 and beta5 strands, which forms part of the central structure of beta-subunit, as the potential binding site of isopenicillin N, which establishes interactions with Cys103, Leu169, Gln172, Asp264, and Arg310, detailed overview Penicillium chrysogenum