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Literature summary extracted from

  • Acero, E.; Ribitsch, D.; Rodriguez, R.; Dellacher, A.; Zitzenbacher, S.; Marold, A.; Greimel, K.; Schroeder, M.; Kandelbauer, A.; Heumann, S.; Nyanhongo, G.; Schwab, H.; Guebitz, G.
    Two-step enzymatic functionalisation of polyamide with phenolics (2012), J. Mol. Catal. B, 79, 54-60.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.13 0.12
-
 4-nitroacetanilide pH 7.0, 25°C Nocardia farcinica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.13 51000
-
 x * 51000, SDS-PAGE, His-tagged protein Nocardia farcinica

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.13 Nocardia farcinica
-
-
-
3.5.1.13 Nocardia farcinica IFM 10152
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.13 immobilized metal ion affinity chromatography (Ni2+) Nocardia farcinica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.13 20
-
 4-nitroacetanilide, pH 7.0, 25°C Nocardia farcinica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.13 4-nitroacetanilide + H2O
-
 Nocardia farcinica 4-nitroaniline + acetate
-
 ?
3.5.1.13 4-nitroacetanilide + H2O
-
 Nocardia farcinica IFM 10152 4-nitroaniline + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.13 ? x * 51000, SDS-PAGE, His-tagged protein Nocardia farcinica

Synonyms

EC Number Synonyms Comment Organism
3.5.1.13 polyamidase
-
 Nocardia farcinica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.13 19.1
-
 4-nitroacetanilide pH 7.0, 25°C Nocardia farcinica