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Literature summary extracted from

  • Fung, A.W.; Ebhardt, H.A.; Abeysundara, H.; Moore, J.; Xu, Z.; Fahlman, R.P.
    An alternative mechanism for the catalysis of peptide bond formation by L/F transferase: substrate binding and orientation (2011), J. Mol. Biol., 409, 617-629.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.6
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.6 D186A inactive Escherichia coli
2.3.2.6 D186E the mutation slightly reduces the reaction rate Escherichia coli
2.3.2.6 D186N inactive Escherichia coli
2.3.2.6 M144A the mutant exhibits about 9% activity compared to the wild type Escherichia coli
2.3.2.6 Q188A the mutant exhibits about 8% activity compared to the wild type Escherichia coli
2.3.2.6 W111A inactive Escherichia coli
2.3.2.6 W49A the mutant exhibits about 10% activity compared to the wild type Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.6 L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide Escherichia coli
-
 tRNA(Leu) + L-phenylalanyl-L-arginyl-protein
-
 ?
2.3.2.6 L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide Escherichia coli
-
 tRNA(Phe) + L-phenylalanyl-L-arginyl-protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.6 Escherichia coli P0A8P1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.6 HisTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.6 L-phenylalanyl-tRNA + REPGLCTWQSLR
-
 Escherichia coli t-RNA + FREPGLCTWQSLR
-
 ?
2.3.2.6 L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide
-
 Escherichia coli tRNA(Leu) + L-phenylalanyl-L-arginyl-protein
-
 ?
2.3.2.6 L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide
-
 Escherichia coli tRNA(Phe) + L-phenylalanyl-L-arginyl-protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.6 L/F transferase
-
 Escherichia coli
2.3.2.6 leucyl/phenylalanyl tRNA protein transferase
-
 Escherichia coli