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Literature summary extracted from

  • Ko, J.A.; Jeong, H.J.; Ryu, Y.B.; Park, S.J.; Wee, Y.J.; Kim, D.; Kim, Y.M.; Lee, W.S.
    Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system (2012), J. Microbiol. Biotechnol., 22, 510-515.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 enzyme expression in the periplasmic space of seven different Escherichia coli strains with inducer molecules such as lactose or IPTG. Escherichia coli strain BL21-CodonPlus(DE3)-RIL exhibits the highest enzyme activity with lactose, method optimization with respect to culture conditions results in a 12fold increase in activity at 17°C and 7.5 mM lactose Leuconostoc citreum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 160000
-
 x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation Leuconostoc citreum
2.4.1.5 167000
-
 x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation Leuconostoc citreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 additional information Leuconostoc citreum DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction ?
-
 ?
2.4.1.5 additional information Leuconostoc citreum KM20 DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction ?
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum KM20
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc citreum B1N0B6
-
-
2.4.1.5 Leuconostoc citreum KM20 B1N0B6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction Leuconostoc citreum ?
-
 ?
2.4.1.5 additional information DexT catalyzes the polymerization of sucrose to dextran via a transglucosyl reaction Leuconostoc citreum KM20 ?
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc citreum D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Leuconostoc citreum D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product analysis by thin layer chromatography ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc citreum KM20 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Leuconostoc citreum KM20 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product analysis by thin layer chromatography ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 160000, recombinant enzyme, SDS-PAGE, x * 167000, about, sequence calculation Leuconostoc citreum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DexT
-
 Leuconostoc citreum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
 recombinant enzyme Leuconostoc citreum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35
-
 purified recombinant enzyme, stable up to Leuconostoc citreum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.5
-
 recombinant enzyme Leuconostoc citreum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 4 7.5 purified recombinant enzyme, stable Leuconostoc citreum

General Information

EC Number General Information Comment Organism
2.4.1.5 evolution dextransucrase belongs to the glycohydrolase family GH 70 Leuconostoc citreum