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Literature summary extracted from

  • Mladkova, J.; Vanek, V.; Budesinsky, M.; Elbert, T.; Demianova, Z.; Garrow, T.A.; Jiracek, J.
    Double-headed sulfur-linked amino acids as first inhibitors for betaine-homocysteine S-methyltransferase 2 (2012), J. Med. Chem., 55, 6822-6831.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.5 expressed in Escherichia coli BL21(DE3) (coexpression of human recombinant betaine-homocysteine S-methyltransferase) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 (2S,11RS)-5-thia-2,11-diamino-8,8-dimethyldodecanedioic acid more than 90% inhibition at 0.02 mM Homo sapiens
2.1.1.5 (2S,11RS)-5-thia-2,11-diaminododecanedioic acid about 60% inhibition at 0.02 mM Homo sapiens
2.1.1.5 (2S,11S)-5,8-dithia-2,11-diaminododecanedioic acid about 60% inhibition at 0.02 mM Homo sapiens
2.1.1.5 (2S,5RS,8RS,11S)-5,8-dithia-2,11-diaminododecanedioic acid 5,8-dioxide about 20% inhibition at 0.02 mM Homo sapiens
2.1.1.5 (2S,8RS,11RS)-5-thia-2,11-diamino-8-methyldodecanedioic acid competitive, more than 90% inhibition at 0.02 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.5 Zn2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.5 40300
-
 x * 40300, 363 amino acid residues Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.5 stabilized by copurification with human recombinant betaine-homocysteine S-methyltransferase, chitin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 kidney
-
 Homo sapiens
-
2.1.1.5 liver
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.5 stable for a minimum of one month at 4°C in a mixture with human recombinant betaine-homocysteine S-methyltransferase (copurified with human recombinant betaine-homocysteine S-methyltransferase) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 L-homocysteine + S-methylmethionine
-
 Homo sapiens 2 L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.5 ? x * 40300, 363 amino acid residues Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.5 betaine-homocysteine S-methyltransferase 2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.5 0.000077
-
 (2S,8RS,11RS)-5-thia-2,11-diamino-8-methyldodecanedioic acid IC50 for betaine-homocysteine S-methyltransferase about 0.077 mM, pH 7.5, 37°C Homo sapiens