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Literature summary extracted from

  • Giacometti, S.; Marrano, C.A.; Bonza, M.C.; Luoni, L.; Limonta, M.; De Michelis, M.I.
    Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana (2012), J. Exp. Bot., 63, 1215-1224.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.10 Calmodulin 0.001 mM calmodulin stimulates the activity of wild type enzyme by about 300% Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.10 mutant enzymes are expressed in Saccharomyces cerevisiae strain K616 (devoid of endogenous Ca2+-ATPases) and His-tagged N-termini of wild type and mutant proteins are expressed in Escherichia coli strain DH5alpha Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.10 S19A the mutant shows 95% of wild type activity Arabidopsis thaliana
7.2.2.10 S19D the mutant with 163% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus Arabidopsis thaliana
7.2.2.10 S22A the mutant shows 70% of wild type activity Arabidopsis thaliana
7.2.2.10 S22D the mutant with wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus Arabidopsis thaliana
7.2.2.10 S27A the mutant shows 127% of wild type activity Arabidopsis thaliana
7.2.2.10 S27D the mutant with 89% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus Arabidopsis thaliana
7.2.2.10 S29A the mutant shows 60% of wild type activity Arabidopsis thaliana
7.2.2.10 S29D the mutant shows 64% of wild type activity Arabidopsis thaliana
7.2.2.10 S57A the mutant shows 50% of wild type activity Arabidopsis thaliana
7.2.2.10 S57D the mutant with 120% of wild type activity is deregulated by showing low activation by calmodulin and tryptic cleavage of the N-terminus. The mutant shows 10fold higher affinity towards calmodulin compared to the wild type enzyme Arabidopsis thaliana
7.2.2.10 S99A the mutant shows 78% of wild type activity Arabidopsis thaliana
7.2.2.10 S99D the mutant with 77% of wild type activity shows half of the wild type affinity towards calmodulin Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.10 plasma membrane
-
 Arabidopsis thaliana 5886
-

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.10 Arabidopsis thaliana Q9LF79
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.2.2.10 phosphoprotein
-
 Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.10 nickel-NTA-agarose column chromatography and glutathione-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.10 ATP + H2O + Ca2+/out
-
 Arabidopsis thaliana ADP + phosphate + Ca2+/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.10 ACA8
-
 Arabidopsis thaliana
7.2.2.10 plasma membrane Ca2+-ATPase
-
 Arabidopsis thaliana