Any feedback?
Literature summary extracted from
- Biochemical characterization of deblocking aminopeptidase from hyperthermophilic archaeon Thermococcus onnurineus NA1 (2007), J. Biosci. Bioeng., 104, 188-194.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.B5 | expression in Escherichia coli | Thermococcus onnurineus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.B5 | Cu2+ | 0.5 mM | Thermococcus onnurineus |  |
| 3.4.11.B5 | EDTA | 0.5 mM, complete inhibition | Thermococcus onnurineus | |
| 3.4.11.B5 | Mn2+ | 0.5 mM | Thermococcus onnurineus | |
| 3.4.11.B5 | Ni2+ | 0.5 mM | Thermococcus onnurineus | |
| 3.4.11.B5 | Zn2+ | 0.5 mM, complete inhibition | Thermococcus onnurineus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.B5 | 0.2 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 0.8 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 1.1 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.B5 | Co2+ | 0.5 mM Co2+ stimulates activity 4.8fold, the enzyme contains 0.09 mol of Co2+ per mol of monomer | Thermococcus onnurineus | |
| 3.4.11.B5 | NaCl | stimulates, optimal cobcentration | Thermococcus onnurineus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 37900 | - |
x * 37900, His-tagged enzyme, SDS-PAGE | Thermococcus onnurineus |
| 3.4.11.B5 | 71000 | - |
dimeric form prevalent in presence of 0.1 mM Co2+, gel filtration | Thermococcus onnurineus |
| 3.4.11.B5 | 600000 | - |
oligomeric form prevalent in absence of Co2+, gel filtration | Thermococcus onnurineus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.B5 | Thermococcus onnurineus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.B5 | - |
Thermococcus onnurineus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.B5 | L-leucyl 4-nitroanilide + H2O | catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide | Thermococcus onnurineus | L-leucine + 4-nitroaniline | - |
? | |
| 3.4.11.B5 | L-methionyl 4-nitroanilide + H2O | - |
Thermococcus onnurineus | L-methionine + 4-nitroaniline | - |
? | |
| 3.4.11.B5 | N-acetyl-L-leucyl 4-nitroanilide + H2O | catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide | Thermococcus onnurineus | N-acetyl-L-leucine + 4-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.B5 | ? | x * 37900, His-tagged enzyme, SDS-PAGE | Thermococcus onnurineus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.B5 | deblocking aminopeptidase | ambiguous | Thermococcus onnurineus |
| 3.4.11.B5 | TNA1-DAP | - |
Thermococcus onnurineus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 90 | 100 | - |
Thermococcus onnurineus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 70 | 100 | 70°C: about 35% of maximal activity, 90-100°C: optimum | Thermococcus onnurineus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 96 | - |
1 h, t1/2 is less than 10 min in 50 mM HEPES buffer (pH 7.5) in absence of CoCl2, stable for at least 1 h in presence of 0.1 mM CoCl2 | Thermococcus onnurineus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.B5 | 0.5 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 10.9 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 37.5 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 7.5 | - |
- |
Thermococcus onnurineus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.B5 | 6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.5: about 70% of maximal activity (HEPES buffer), about 40% of maximal activity (Tris-HCl buffer) | Thermococcus onnurineus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.B5 | 0.45 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 47 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 3.4.11.B5 | 55 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
