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Literature summary extracted from

  • Shimizu, T.; Lin, F.; Hasegawa, M.; Okada, K.; Nojiri, H.; Yamane, H.
    Purification and identification of naringenin 7-O-methyltransferase, a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice (2012), J. Biol. Chem., 287, 19315-19325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.232 gene Os12g0240900, DNA and amino acid sequence determination and analysis, expression of GST-tagged NOMT in Escherichia coli strain Rosetta II (DE3) Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.232 additional information
-
 additional information kinetic analysis, overview Oryza sativa
2.1.1.232 0.0019
-
 (2S)-naringenin pH 8.5, 30°C, purified recombinant GST-tagged NOMT Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin Oryza sativa
-
 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.232 Oryza sativa I2FFE9 var. japonica and cv. Nipponbare, gene Os12g0240900
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.232 purification of native enzyme 400fold from caffeic acid 3-O-methyltransferase-deficient oscomt1 mutant rice plant leaves induced with UV-light by ammonium sulfate fractionation and adenosine affinity chromatography, recombinant GST-tagged NOMT from Escherichia coli strain Rosetta II (DE3) by glutathione affinity chromatography and ultrafiltration Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.232 leaf
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.232 0.0036
-
 purified NOMT, pH 8.5, 28°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.232 additional information the enzyme is also active on kaempferol, apigenin, luteolin, liquiritigenin, and quercetin Oryza sativa ?
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Oryza sativa S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Oryza sativa S-adenosyl-L-homocysteine + (2S)-sakuranetin mass spectrometric and chiral analysis of sakuranetin product ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.232 NOMT
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.232 28 30 assay at Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.232 25
-
 (2S)-naringenin pH 8.5, 30°C, purified recombinant GST-tagged NOMT Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.232 8.5
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.232 S-adenosyl-L-methionine
-
 Oryza sativa

Expression

EC Number Organism Comment Expression
2.1.1.232 Oryza sativa enzyme expression is inducible by by ultraviolet irradiation, CuCl2 treatment, jasmonic acid treatment, and infection by phytopathogens, e.g. fungus Magnaporthe oryzae up

General Information

EC Number General Information Comment Organism
2.1.1.232 metabolism naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice Oryza sativa
2.1.1.232 additional information sakuranetin has anti-inflammatory activity, anti-mutagenic activity, anti-pathogenic activities against Helicobacter pylori, Leishmania, and Trypanosoma and contributes to the maintenance of glucose homeostasis in animals Oryza sativa
2.1.1.232 physiological function naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of major flavonoid phytoalexin sakuranetin in rice, sakuranetin is a useful compound as a plant antibiotic and a potential pharmaceutical agent Oryza sativa