EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.26 | 7-oxocholesterol | - |
Homo sapiens | |
2.3.1.26 | cholesterol | acts as a strong activator and as a substrate | Homo sapiens | |
2.3.1.26 | sitosterol | - |
Homo sapiens | |
2.3.1.26 | sitosterol | weak activator | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.26 | expressed as a His-tagged fusion protein in Escherichia coli | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.26 | F12511 | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.26 | 0.0012 | - |
pregnenolone | pH 7.4, 37°C, with cholesterol (0.32 mM). Without cholesterol, the Km for pregnenolone is too high to be measurable | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.26 | Homo sapiens | O75908 | - |
- |
2.3.1.26 | Homo sapiens | P35610 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.26 | acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
2.3.1.26 | pregnenolone + oleoyl-CoA | without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for PREG esterification increases by 100fold | Homo sapiens | 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA | - |
? | |
2.3.1.26 | pregnenolone + oleoyl-CoA | without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for pregnenolone esterification increases by 100fold | Homo sapiens | 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.26 | ACAT1 | - |
Homo sapiens |
2.3.1.26 | ACAT2 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.26 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.26 | 7.4 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.26 | malfunction | mice lacking ACAT1 or ACAT2 do not have a decreased PREG ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum | Homo sapiens |
2.3.1.26 | malfunction | mice lacking ACAT1 or ACAT2 do not have a decreased pregnenolone ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum | Homo sapiens |