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Literature summary extracted from

  • Rogers, M.A.; Liu, J.; Kushnir, M.M.; Bryleva, E.; Rockwood, A.L.; Meikle, A.W.; Shapiro, D.; Vaisman, B.L.; Remaley, A.T.; Chang, C.C.; Chang, T.Y.
    Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase (2012), J. Biol. Chem., 287, 17483-17492.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 7-oxocholesterol
-
Homo sapiens
2.3.1.26 cholesterol acts as a strong activator and as a substrate Homo sapiens
2.3.1.26 sitosterol
-
Homo sapiens
2.3.1.26 sitosterol weak activator Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 expressed as a His-tagged fusion protein in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 F12511
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.26 0.0012
-
pregnenolone pH 7.4, 37°C, with cholesterol (0.32 mM). Without cholesterol, the Km for pregnenolone is too high to be measurable Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Homo sapiens O75908
-
-
2.3.1.26 Homo sapiens P35610
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
Homo sapiens CoA + cholesterol ester
-
?
2.3.1.26 pregnenolone + oleoyl-CoA without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for PREG esterification increases by 100fold Homo sapiens 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA
-
?
2.3.1.26 pregnenolone + oleoyl-CoA without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for pregnenolone esterification increases by 100fold Homo sapiens 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.26 ACAT1
-
Homo sapiens
2.3.1.26 ACAT2
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.26 7.4
-
assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.26 malfunction mice lacking ACAT1 or ACAT2 do not have a decreased PREG ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum Homo sapiens
2.3.1.26 malfunction mice lacking ACAT1 or ACAT2 do not have a decreased pregnenolone ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum Homo sapiens